Structure of dihydrodipicolinate synthase from Methanocaldococcus jannaschii

Structure of dihydrodipicolinate synthase from Methanocaldococcus jannaschii In bacteria and plants, dihydrodipicolinate synthase (DHDPS) plays a key role in the (S)‐lysine biosynthesis pathway. DHDPS catalyzes the first step of the condensation of (S)‐aspartate‐β‐semialdehyde and pyruvate to form an unstable compound, (4S)‐4‐hydroxy‐2,3,4,5‐tetrahydro‐(2S)‐dipicolinic acid. The activity of DHDPS is allosterically regulated by (S)‐lysine, a feedback inhibitor. The crystal structure of DHDPS from Methanocaldococcus jannaschii (MjDHDPS) was solved by the molecular‐replacement method and was refined to 2.2 Å resolution. The structure revealed that MjDHDPS forms a functional homotetramer, as also observed in Escherichia coli DHDPS, Thermotoga maritima DHDPS and Bacillus anthracis DHDPS. The binding‐site region of MjDHDPS is essentially similar to those found in other known DHDPS structures. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

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Publisher
Wiley
Copyright
International Union of Crystallography, 2009
ISSN
1744-3091
eISSN
1744-3091
D.O.I.
10.1107/S174430910904651X
Publisher site
See Article on Publisher Site

Abstract

In bacteria and plants, dihydrodipicolinate synthase (DHDPS) plays a key role in the (S)‐lysine biosynthesis pathway. DHDPS catalyzes the first step of the condensation of (S)‐aspartate‐β‐semialdehyde and pyruvate to form an unstable compound, (4S)‐4‐hydroxy‐2,3,4,5‐tetrahydro‐(2S)‐dipicolinic acid. The activity of DHDPS is allosterically regulated by (S)‐lysine, a feedback inhibitor. The crystal structure of DHDPS from Methanocaldococcus jannaschii (MjDHDPS) was solved by the molecular‐replacement method and was refined to 2.2 Å resolution. The structure revealed that MjDHDPS forms a functional homotetramer, as also observed in Escherichia coli DHDPS, Thermotoga maritima DHDPS and Bacillus anthracis DHDPS. The binding‐site region of MjDHDPS is essentially similar to those found in other known DHDPS structures.

Journal

Acta Crystallographica Section FWiley

Published: Dec 1, 2009

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