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L. Vindeløv, I. Christensen, Niels Keiding, M. Spang‐Thomsen, N. Nissen (1983)
Long-term storage of samples for flow cytometric DNA analysis.Cytometry, 3 5
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Flow cytometric analysis of DNA content for ploidy determination in human solid tumors.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 27
L. Vindeløv, I. Christensen, N. Nissen (1983)
A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis.Cytometry, 3 5
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Limits of detection of nuclear DNA abnormalities by flow cytometric DNA analysis. Results obtained by a set of methods for sample-storage, staining and internal standardization.Cytometry, 3 5
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Determination of nuclear DNA content by flow cytometry requires comparison with a reference standard. The use of external standards such as lymphocytes or granulocytes is time‐consuming and inaccurate. Chicken red blood cells (CRBC) have a DNA content of 35% of the human diploid value and have been widely used as internal standard. The ratio calculated on the basis of the peak channel numbers of the standard and the sample and used to indicate the DNA content (DNA ratio) is, however, very sensitive to changes in the zero level adjustment of the flow cytometer. If two internal standards are used the DNA ratio becomes independent of the zero level. Rainbow trout red blood cells (TRBC) have a DNA content of 80% of human diploid cells. A mixture of CRBC and TRBC was prepared and stored in small aliquots at —80°C. This mixture was added to the sample before staining. The day‐to‐day variation of the DNA ratio obtained by use of the two standards was smaller than that obtained by CRBC alone. The possibility of sex related differences in DNA content of CRBC and TRBC was examined. The results indicated that a new batch of standards should be tested against the old batch to avoid the introduction of a systematic error.
Cytometry Part A – Wiley
Published: Mar 1, 1983
Keywords: ; ; ; ;
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