Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta Summary Stable genetic transformation of Arabidopsis thaliana was achieved by simple in planta inoculation of Agrobacterium tumefaciens strain LBA4404 harboring a binary vector pBl121. The transformation procedure, which we call in planta transformation, involves severing of apical shoots at their bases, inoculation with Agrobacterium at the severed sites, and in planta generation of shoots from the severed sites. On average, 5.5% of the newly formed shoots produced transformed progenies. These progenies (T2 generation) contained T‐DNA in the genome as examined by assaying the T‐DNA encoded β‐glucuronidase and kanamycin resistance and by genomic Southern blot analysis, the copy number of the T‐DNAs in the Arabidopsis genome being single (33%) or multiple. The genetic behavior of the transformants examined at the T3 and T4 generations or with the F2 progenies of the outcrosses between transformants and wild‐type plants showed that most of the inserted T‐DNA are inherited in a Mendelian fashion. This procedure provides a new approach for simple and efficient transformation of A. thaliana, obviating the need for plant regeneration from tissue explants in vitro. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

Loading next page...
 
/lp/wiley/stable-genetic-transformation-of-arabidopsis-thaliana-by-agrobacterium-chwRBTXe20
Publisher
Wiley
Copyright
Copyright © 1994 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
D.O.I.
10.1046/j.1365-313X.1994.05040551.x
Publisher site
See Article on Publisher Site

Abstract

Summary Stable genetic transformation of Arabidopsis thaliana was achieved by simple in planta inoculation of Agrobacterium tumefaciens strain LBA4404 harboring a binary vector pBl121. The transformation procedure, which we call in planta transformation, involves severing of apical shoots at their bases, inoculation with Agrobacterium at the severed sites, and in planta generation of shoots from the severed sites. On average, 5.5% of the newly formed shoots produced transformed progenies. These progenies (T2 generation) contained T‐DNA in the genome as examined by assaying the T‐DNA encoded β‐glucuronidase and kanamycin resistance and by genomic Southern blot analysis, the copy number of the T‐DNAs in the Arabidopsis genome being single (33%) or multiple. The genetic behavior of the transformants examined at the T3 and T4 generations or with the F2 progenies of the outcrosses between transformants and wild‐type plants showed that most of the inserted T‐DNA are inherited in a Mendelian fashion. This procedure provides a new approach for simple and efficient transformation of A. thaliana, obviating the need for plant regeneration from tissue explants in vitro.

Journal

The Plant JournalWiley

Published: Apr 1, 1994

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off