Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth‐promoting activities

Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine... We established spontaneously immortalized Schwann cell lines from long‐term cultures of adult mouse dorsal root ganglia and peripheral nerves. One of the cell lines, designated IMS32, responded to mitogenic stimuli by platelet‐derived growth factor (PDGF)‐BB, acidic and basic fibroblast growth factors (aFGF, bFGF), and transforming growth factors (TGF)‐b̃1 and ‐b̃2, as determined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for S100 and BrdU. Furthermore, conditioned media (CM) obtained from IMS32 cells showed mitogenic activity for both IMS32 cells and long‐term cultured Schwann cells. Western blot analysis revealed TGF‐b̃‐like molecule in the CM, and the activity was absorbed with anti‐TGF‐b̃ neutralizing antibody. Reverse transcription followed by polymerase chain reaction (RT‐PCR) of IMS32 RNA revealed that these cells expressed TGF‐b̃1, b̃2, and ‐b̃3 transcripts. When rat pheochromocytoma PC 12 cells were incubated with the CM, they developed neurite growth. Coculture of PC12 and IMS32 cells also showed neurite growth of PC12 cells. RNA transcripts of nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF), neurotrophin‐3 (NT3), ciliary neurotrophic factor (CNTF), and glial cell line‐derived neurotrophic factor (GDNF) were detected from IMS32 cells by RT‐PCR. In these, we sequenced the mouse GDNF cDNA coding region and observed 97% and 90% homologies to corresponding rat and human cDNA sequences, respectively. These results indicate that the immortalized Schwann cell line mitotically responds to various growth factors and secretes autocrine and paracrine growth‐promoting activities in vitro. © 1995 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth‐promoting activities

Loading next page...
 
/lp/wiley/spontaneously-immortalized-adult-mouse-schwann-cells-secrete-autocrine-Xi4n0GU7Wj
Publisher
Wiley
Copyright
Copyright © 1995 Wiley‐Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
D.O.I.
10.1002/jnr.490410215
Publisher site
See Article on Publisher Site

Abstract

We established spontaneously immortalized Schwann cell lines from long‐term cultures of adult mouse dorsal root ganglia and peripheral nerves. One of the cell lines, designated IMS32, responded to mitogenic stimuli by platelet‐derived growth factor (PDGF)‐BB, acidic and basic fibroblast growth factors (aFGF, bFGF), and transforming growth factors (TGF)‐b̃1 and ‐b̃2, as determined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for S100 and BrdU. Furthermore, conditioned media (CM) obtained from IMS32 cells showed mitogenic activity for both IMS32 cells and long‐term cultured Schwann cells. Western blot analysis revealed TGF‐b̃‐like molecule in the CM, and the activity was absorbed with anti‐TGF‐b̃ neutralizing antibody. Reverse transcription followed by polymerase chain reaction (RT‐PCR) of IMS32 RNA revealed that these cells expressed TGF‐b̃1, b̃2, and ‐b̃3 transcripts. When rat pheochromocytoma PC 12 cells were incubated with the CM, they developed neurite growth. Coculture of PC12 and IMS32 cells also showed neurite growth of PC12 cells. RNA transcripts of nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF), neurotrophin‐3 (NT3), ciliary neurotrophic factor (CNTF), and glial cell line‐derived neurotrophic factor (GDNF) were detected from IMS32 cells by RT‐PCR. In these, we sequenced the mouse GDNF cDNA coding region and observed 97% and 90% homologies to corresponding rat and human cDNA sequences, respectively. These results indicate that the immortalized Schwann cell line mitotically responds to various growth factors and secretes autocrine and paracrine growth‐promoting activities in vitro. © 1995 Wiley‐Liss, Inc.

Journal

Journal of Neuroscience ResearchWiley

Published: Jun 1, 1995

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off