The extractability of spectrin from red blood cell membranes was measured both by dialysis against 1 mM EDTA for 44 hours and by incubation with 1 mM EDTA for 60 minutes. We confirmed that the extractability of spectrin is somewhat impaired when membranes are prepared from metabolically depleted erythrocytes, but the extent of impairment was considerably < has been previously reported. Red blood cells which had been rendered nonviable by prolonged storage in citrate phosphate dextrose solution manifested normal spectrin extractability. We conclude that the loss of spectrin extractability is an insensitive manifestation of loss of red blood cell viability, and that it is not suitable for the prediction of loss of viability during red blood cell storage.
Transfusion – Wiley
Published: Jan 2, 1981
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