Sonic hedgehog and tiggy‐winkle hedgehog cooperatively induce zebrafish branchiomotor neurons

Sonic hedgehog and tiggy‐winkle hedgehog cooperatively induce zebrafish branchiomotor neurons Stephanie Bingham1, Aidas Nasevicius2, Stephen C. Ekker2, and Anand Chandrasekhar1, Division of Biological Sciences, University of Missouri, Columbia, Missouri, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota Received 1 May 2001; Accepted 12 June 2001 Published online 23 July 2001; DOI 10.1002/gene.1057 The secreted molecule sonic hedgehog (Shh) is essential for many developmental processes in vertebrates, including the induction of motor neurons (reviewed in Ingham, 1998; Wicking et al., 1999). Three hedgehog genes, shh (Krauss et al., 1993), tiggy-winkle hedgehog (twhh; Ekker et al., 1995) and echidna hedgehog (ehh; Currie and Ingham, 1996) are expressed in various tissues in zebrafish embryos. However, only mutations in shh have been identified thus far (Schauerte et al., 1998). Therefore, the precise roles of the three zebrafish hedgehog genes in inducing particular cell types such as motor neurons remain unclear (Beattie et al., 1997; Chandrasekhar et al., 1998). We showed previously that embryos homozygous for a deletion of shh (Schauerte et al., 1998) exhibit characteristic deficits in branchiomotor neuron (BMN) populations in the zebrafish hindbrain (Chandrasekhar et al., 1998). We now demonstrate that knockdown of shh function by morpholino (MO) injection phenocopies the shh loss-of-function motor neuron phenotype. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genesis: the Journal of Genetics and Development Wiley

Sonic hedgehog and tiggy‐winkle hedgehog cooperatively induce zebrafish branchiomotor neurons

Loading next page...
 
/lp/wiley/sonic-hedgehog-and-tiggy-winkle-hedgehog-cooperatively-induce-bKDjd276sC
Publisher
Wiley
Copyright
Copyright © 2001 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1526-954X
eISSN
1526-968X
DOI
10.1002/gene.1057
Publisher site
See Article on Publisher Site

Abstract

Stephanie Bingham1, Aidas Nasevicius2, Stephen C. Ekker2, and Anand Chandrasekhar1, Division of Biological Sciences, University of Missouri, Columbia, Missouri, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota Received 1 May 2001; Accepted 12 June 2001 Published online 23 July 2001; DOI 10.1002/gene.1057 The secreted molecule sonic hedgehog (Shh) is essential for many developmental processes in vertebrates, including the induction of motor neurons (reviewed in Ingham, 1998; Wicking et al., 1999). Three hedgehog genes, shh (Krauss et al., 1993), tiggy-winkle hedgehog (twhh; Ekker et al., 1995) and echidna hedgehog (ehh; Currie and Ingham, 1996) are expressed in various tissues in zebrafish embryos. However, only mutations in shh have been identified thus far (Schauerte et al., 1998). Therefore, the precise roles of the three zebrafish hedgehog genes in inducing particular cell types such as motor neurons remain unclear (Beattie et al., 1997; Chandrasekhar et al., 1998). We showed previously that embryos homozygous for a deletion of shh (Schauerte et al., 1998) exhibit characteristic deficits in branchiomotor neuron (BMN) populations in the zebrafish hindbrain (Chandrasekhar et al., 1998). We now demonstrate that knockdown of shh function by morpholino (MO) injection phenocopies the shh loss-of-function motor neuron phenotype.

Journal

Genesis: the Journal of Genetics and DevelopmentWiley

Published: Jul 1, 2001

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off