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Solvent selection for enhanced bioproduction of 3‐methylcatechol in a two‐phase partitioning bioreactor

Solvent selection for enhanced bioproduction of 3‐methylcatechol in a two‐phase partitioning... The biotransformation of toluene to 3‐methycatechol (3MC) via Pseudomonas putida MC2 was used as a model system for the development of a biphasic process offering enhanced overall volumetric productivity. Three factors were investigated for the identification of an appropriate organic solvent and they included solvent toxicity, bioavailability of the solvent as well as solvent affinity for 3MC. The critical log P (log Pcrit) of the biocatalyst was found to be 3.1 and log P values were used to predict a solvent's toxicity. The presence of various functional groups of candidate solvents were used to predict the absorption of 3MC and it was found that solvents possessing polarity showed an affinity towards 3MC. Bis (2‐ethylhexyl) sebecate was selected for use in the biphasic system as it fulfilled all selection criteria. A two‐phase biotransformation with BES and a 50% phase volume ratio, achieved an overall volumetric productivity of 440 mg 3MC/L‐h, which was an improvement by a factor of approximately 4 over previously operated systems. Additional work focused on reducing the toluene feed in order to minimize possible toxicity and decrease loss of substrate (toluene), a result of volatilization. Toluene losses were reduced by a factor of 4, compared to previously operated systems, without suffering an appreciable loss in overall volumetric productivity. Biotechnol. Bioeng. 2007;97: 536–543. © 2006 Wiley Periodicals, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

Solvent selection for enhanced bioproduction of 3‐methylcatechol in a two‐phase partitioning bioreactor

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References (38)

Publisher
Wiley
Copyright
Copyright © 2007 Wiley Periodicals, Inc., A Wiley Company
ISSN
0006-3592
eISSN
1097-0290
DOI
10.1002/bit.21257
pmid
17099912
Publisher site
See Article on Publisher Site

Abstract

The biotransformation of toluene to 3‐methycatechol (3MC) via Pseudomonas putida MC2 was used as a model system for the development of a biphasic process offering enhanced overall volumetric productivity. Three factors were investigated for the identification of an appropriate organic solvent and they included solvent toxicity, bioavailability of the solvent as well as solvent affinity for 3MC. The critical log P (log Pcrit) of the biocatalyst was found to be 3.1 and log P values were used to predict a solvent's toxicity. The presence of various functional groups of candidate solvents were used to predict the absorption of 3MC and it was found that solvents possessing polarity showed an affinity towards 3MC. Bis (2‐ethylhexyl) sebecate was selected for use in the biphasic system as it fulfilled all selection criteria. A two‐phase biotransformation with BES and a 50% phase volume ratio, achieved an overall volumetric productivity of 440 mg 3MC/L‐h, which was an improvement by a factor of approximately 4 over previously operated systems. Additional work focused on reducing the toluene feed in order to minimize possible toxicity and decrease loss of substrate (toluene), a result of volatilization. Toluene losses were reduced by a factor of 4, compared to previously operated systems, without suffering an appreciable loss in overall volumetric productivity. Biotechnol. Bioeng. 2007;97: 536–543. © 2006 Wiley Periodicals, Inc.

Journal

Biotechnology and BioengineeringWiley

Published: Jun 15, 2007

Keywords: biocatalysis; solvent selection; two‐phase; 3‐methylcatechol

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