Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a... A selective and sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for investigating the pharmacokinetics of umbelliferone, apigenin, genkwanin and hydroxygenkwanin after oral administration of Daphne genkwa extract. Plasma samples were treated by protein precipitation with acetonitrile. Analytes were detected by triple‐quadrupole MS/MS with an ESI source in negative selection reaction monitoring mode. The transitions of m/z 161 → 133 for umbelliferone, m/z 269 → 117 for apigenin, m/z 283 → 268 for genkwanin and m/z 299 → 284 for hydroxygenkwanin were confirmed for quantification. Chromatographic separation was conducted using an Eclipse XDB‐C18 column, and the applied isocratic elution program allowed for simultaneous determination of the four analytes for a total run time of 2.5 min. The linearity was validated over the plasma concentration ranges of 1.421–1421 ng/mL for umbelliferone, 0.845–845 ng/mL for apigenin, 1.025–1025 ng/mL for genkwanin and 0.845–845 ng/mL for hydroxygenkwanin. The extraction recovery rate was >82.7% for each analyte. No apparent matrix effect was observed during the bioanalysis. After full validation, the proposed method was successfully applied to compare the pharmacokinetics of these analytes between normal and arthritic rats. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biomedical Chromatography Wiley

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Zhou, Luyi; Li, Jing; Yan, Chong
Biomedical Chromatography, Volume 32 (7) – Jan 1, 2018
Download PDF
10 pages
Read Article

Loading next page...
 
/lp/wiley/simultaneous-determination-of-three-flavonoids-and-one-coumarin-by-lc-mcyXUqa8fz
Publisher
Wiley
Copyright
Copyright © 2018 John Wiley & Sons, Ltd.
ISSN
0269-3879
eISSN
1099-0801
DOI
10.1002/bmc.4233
pmid
29500935
Publisher site
See Article on Publisher Site

Abstract

A selective and sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for investigating the pharmacokinetics of umbelliferone, apigenin, genkwanin and hydroxygenkwanin after oral administration of Daphne genkwa extract. Plasma samples were treated by protein precipitation with acetonitrile. Analytes were detected by triple‐quadrupole MS/MS with an ESI source in negative selection reaction monitoring mode. The transitions of m/z 161 → 133 for umbelliferone, m/z 269 → 117 for apigenin, m/z 283 → 268 for genkwanin and m/z 299 → 284 for hydroxygenkwanin were confirmed for quantification. Chromatographic separation was conducted using an Eclipse XDB‐C18 column, and the applied isocratic elution program allowed for simultaneous determination of the four analytes for a total run time of 2.5 min. The linearity was validated over the plasma concentration ranges of 1.421–1421 ng/mL for umbelliferone, 0.845–845 ng/mL for apigenin, 1.025–1025 ng/mL for genkwanin and 0.845–845 ng/mL for hydroxygenkwanin. The extraction recovery rate was >82.7% for each analyte. No apparent matrix effect was observed during the bioanalysis. After full validation, the proposed method was successfully applied to compare the pharmacokinetics of these analytes between normal and arthritic rats.

Journal

Biomedical Chromatography – Wiley

Published: Jan 1, 2018

Keywords: ; ; ;

References

  • Quantitative determination of betamethasone sodium phosphate and betamethasone dipropionate in human plasma by UPLC‐MS/MS and a bioequivalence study
    Chen, M. Y.; Tang, Y. J.; Wang, Y. C.; Wang, C. Z.; Yuan, C. S.; Chen, Y.; Zhou, H. H.
  • Apigenin potentiates the antitumor activity of 5‐FU on solid Ehrlich carcinoma: Crosstalk between apoptotic and JNK‐mediated autophagic cell death platforms
    Gaballah, H. H.; Gaber, R. A.; Mohamed, D. A.
  • Growth inhibition of human lung cancer cells via down‐regulation of epidermal growth factor receptor signaling by yuanhuadine, a daphnane diterpene from Daphne genkwa
    Hong, J. Y.; Chung, H. J.; Lee, H. J.; Park, H. J.; Lee, S. K.
  • Daphnane‐type diterpene esters with cytotoxic and anti‐HIV‐1 activities from Daphne acutiloba Rehd
    Huang, S. Z.; Zhang, X. J.; Li, X. Y.; Kong, L. M.; Jiang, H. Z.; Ma, Q. Y.; Zhao, Y. X.
  • The Encyclopedia of Traditional Chinese Medicine
  • Anti‐rheumatoid arthritic activity of flavonoids from Daphne genkwa
    Jiang, C. P.; He, X.; Yang, X. L.; Zhang, S. L.; Li, H.; Song, Z. J.; Yuan, C. S.
  • Apigenin isolated from Daphne genkwa Siebold et Zucc. inhibits 3T3‐L1 preadipocyte differentiation through a modulation of mitotic clonal expansion
    Kim, M. A.; Kang, K.; Lee, H. J.; Kim, M.; Kim, C. Y.; Nho, C. W.
  • Immunomodulatory and cellular antioxidant activities of pure compounds from Teucrium ramosissimum Desf
    Nasr‐Bouzaiene, N.; Sassi, A.; Bedoui, A.; Krifa, M.; Chekir‐Ghedira, L.; Ghedira, K.
  • Antifungal activity of umbelliferone derivatives: Synthesis and structure‐activity relationships
    Pan, L.; Li, X.; Jin, H.; Yang, X.; Qin, B.
  • Anti‐inflammatory effect of apigenin on LPS‐induced pro‐inflammatory mediators and AP‐1 factors in human lung epithelial cells
    Patil, R. H.; Babu, R. L.; Naveen Kumar, M.; Kiran Kumar, K. M.; Hegde, S. M.; Nagesh, R.; Sharma, S. C.
  • Determination of genkwanin in rat plasma by liquid chromatography‐tandem mass spectrometry: Application to a bioavailability study
    Song, Y.; Zhang, S.; Liu, H.; Jin, X.
  • Chinese Pharmacopoeia I
  • Simultaneous determination of four flavonoids and one phenolic acid in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study after oral administration of the Herba Desmodii Styracifolii extract
    Sun, D.; Dong, L.; Guo, P.; Yan, W.; Wang, C.; Zhang, Z.
  • Preventive effect of grape seed extract against high‐fructose diet‐induced insulin resistance and oxidative stress in rats
    Suwannaphet, W.; Meeprom, A.; Yibchok‐Anun, S.; Adisakwattana, S.
  • Functional restoration of exhausted CD4(+) and CD8(+) T cells in chronic viral infection by vinegar‐processed flos of Daphne genkwa
    Uyangaa, E.; Choi, J. Y.; Patil, A. M.; Kim, J. H.; Kim, S. B.; Kim, K.; Eo, S. K.
  • Comparative pharmacokinetic study of two boswellic acids in normal and arthritic rat plasma after oral administration of Boswellia serrata extract or Huo Luo Xiao Ling Dan by LC‐MS
    Wang, H.; Zhang, C.; Wu, Y.; Ai, Y.; Lee, D. Y.; Dai, R.
  • Antitumor and immunomodulatory activity of genkwanin on colorectal cancer in the APC(Min/+) mice
    Wang, X.; Song, Z. J.; He, X.; Zhang, R. Q.; Zhang, C. F.; Li, F.; Yuan, C. S.
  • Synergistic anti‐glioma effect of hydroxygenkwanin and apigenin in vitro
    Wang, Y.; Xu, Y. S.; Yin, H. L.; Xu, L. N.; Peng, J. Y.; Zhou, H.; Kang, W.
  • An UPLC‐MS/MS method for simultaneous quantitation of two coumarins and two flavonoids in rat plasma and its application to a pharmacokinetic study of Wikstroemia indica extract
    Wei, L.; Wang, X.; Zhang, P.; Sun, Y.; Jia, L.; Zhao, J.; Sun, L.
  • Simultaneous determination of seven coumarins by UPLC‐MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Huo Luo Xiao Ling Dan or single‐herb extract
    Wu, Y.; Wang, F.; Ai, Y.; Ma, W.; Bian, Q.; Lee, D. Y.; Dai, R.
  • Apigenin suppresses colorectal cancer cell proliferation, migration and invasion via inhibition of the Wnt/β‐catenin signaling pathway
    Xu, M.; Wang, S.; Song, Y. U.; Yao, J.; Huang, K.; Zhu, X.
  • Antioxidant effects of Genkwa flos flavonoids on Freund's adjuvant‐induced rheumatoid arthritis in rats
    Zhang, C. F.; Zhang, S. L.; He, X.; Yang, X. L.; Wu, H. T.; Lin, B. Q.; Yuan, C. S.
  • Simultaneous determination of seven flavonoids and one coumarin of Genkwa Flos by RP‐HPLC
    Zhang, W.; Peng, Y.; Huang, Y.; Li, L.; Song, S.; Bi, K.
  • LC‐MS/MS determination and pharmacokinetic study of seven flavonoids in rat plasma after oral administration of Cirsium japonicum DC. Extract
    Zhang, Z.; Jia, P.; Zhang, X.; Zhang, Q.; Yang, H.; Shi, H.; Zhang, L.
  • Simultaneous determination of nine coumarins in rat plasma by HPLC‐MS/MS for pharmacokinetics studies following oral administration of Fraxini Cortex extract
    Zhao, M.; Ding, W.; Wang, S.; Wang, C.; Du, Y.; Xu, H.; Jin, S.
  • Pharmacokinetic study of apigenin, 3‐hydroxy‐genkwanin and genkwanin in rats after oral administration of extraction of Dephne Genkwa Sieb.et Zucc by LC‐MS/MS
    Zheng, L.; Yin, L.; Xu, L.; Wang, Y.

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial