Simultaneous determination of residues of thiamethoxam and its metabolite clothianidin in tobacco leaf and soil using liquid chromatography‐tandem mass spectrometry

Simultaneous determination of residues of thiamethoxam and its metabolite clothianidin in tobacco... A simple analytical method was developed to simultaneously determine thiamethoxam and its metabolite, clothianidin, in fresh tobacco leaf, soil and cured tobacco leaf using liquid chromatography with tandem mass spectrometry. Thiamethoxam and clothianidin in tobacco and soil samples were extracted with acetonitrile containing 0.1% formic acid and purified using an NH2‐SPE column. The optimized method provided good linearity with coefficients of determination R2 ≥ 0.9981. The limits of detection and quantification were between 0.006–0.12 and 0.02–0.4 mg/kg, respectively. Intra‐ and inter‐day recovery assays were used to validate the established method. The average recoveries of thiamethoxam and clothianidin in fresh tobacco leaf, soil and cured tobacco leaf were 75.04–100.47%, 75.86–86.40% and 89.83–99.39%, respectively. The intra‐ and inter‐day relative standard deviations were all <9%. The developed method was successfully applied for the analysis of thiamethoxam and clothianidin residues in actual tobacco and soil samples. The results indicated that the established method met the requirements for the analysis of trace amounts of thiamethoxam and clothianidin in fresh tobacco leaf, soil and cured tobacco leaf. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biomedical Chromatography Wiley

Simultaneous determination of residues of thiamethoxam and its metabolite clothianidin in tobacco leaf and soil using liquid chromatography‐tandem mass spectrometry

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Publisher
Wiley
Copyright
Copyright © 2018 John Wiley & Sons, Ltd.
ISSN
0269-3879
eISSN
1099-0801
D.O.I.
10.1002/bmc.4225
Publisher site
See Article on Publisher Site

Abstract

A simple analytical method was developed to simultaneously determine thiamethoxam and its metabolite, clothianidin, in fresh tobacco leaf, soil and cured tobacco leaf using liquid chromatography with tandem mass spectrometry. Thiamethoxam and clothianidin in tobacco and soil samples were extracted with acetonitrile containing 0.1% formic acid and purified using an NH2‐SPE column. The optimized method provided good linearity with coefficients of determination R2 ≥ 0.9981. The limits of detection and quantification were between 0.006–0.12 and 0.02–0.4 mg/kg, respectively. Intra‐ and inter‐day recovery assays were used to validate the established method. The average recoveries of thiamethoxam and clothianidin in fresh tobacco leaf, soil and cured tobacco leaf were 75.04–100.47%, 75.86–86.40% and 89.83–99.39%, respectively. The intra‐ and inter‐day relative standard deviations were all <9%. The developed method was successfully applied for the analysis of thiamethoxam and clothianidin residues in actual tobacco and soil samples. The results indicated that the established method met the requirements for the analysis of trace amounts of thiamethoxam and clothianidin in fresh tobacco leaf, soil and cured tobacco leaf.

Journal

Biomedical ChromatographyWiley

Published: Jan 1, 2018

Keywords: ; ; ;

References

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