Simultaneous Determination of 3,4‐Dihydroxyphenylalanine, 5‐Hydroxytryptophan, Dopamine, 4‐Hydroxy‐3‐Methoxyphenylalanine, Norepinephrine, 3,4‐Dihydroxyphenylacetic Acid, Homovanillic Acid, Serotonin, and 5‐Hydroxyindoleacetic Acid in Rat Cerebrospinal Fluid and Brain by High‐Performance Liquid Chromatography with Electrochemical Detection

Simultaneous Determination of 3,4‐Dihydroxyphenylalanine, 5‐Hydroxytryptophan, Dopamine,... Abstract: A method using reversed‐phase ion‐pair high‐performance liquid chromatography with electrochemical detection for the simultaneous determination of tryptophan (TRP), 3,4‐dihydroxyphenylalanine (DOPA), and their metabolites in whole brain, small‐brain parts, and cerebrospinal fluid of rats has been developed. The sample preparation requires only homogenization in perchloric acid and centrifugation before injection onto the column. With a LiChrosorb RP‐18 (10 μm) column and a mobile phase consisting of a phosphate (NaH2PO4, 0.1 M)‐methanol mixture with octylsulfonate (2.6 × 10−3M) at pH 3.35 and 26°C, the separation of DOPA, dopamine, norepinephrine, 3,4‐dihydroxyphenylacetic acid, homovanillic acid, 4‐hydroxy‐3‐methoxyphenylalanine, TRP, 5‐hydroxytryptophan (SHTP), serotonin, and 5‐hydroxyindole‐acetic acid was achieved. The method has been applied to study the effect of α‐monofluoromethyldopa alone and in combination with L‐DOPA or L‐5‐HTP, on the catechol and 5‐OH indole levels in brain and CSF of the rat. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Simultaneous Determination of 3,4‐Dihydroxyphenylalanine, 5‐Hydroxytryptophan, Dopamine, 4‐Hydroxy‐3‐Methoxyphenylalanine, Norepinephrine, 3,4‐Dihydroxyphenylacetic Acid, Homovanillic Acid, Serotonin, and 5‐Hydroxyindoleacetic Acid in Rat Cerebrospinal Fluid and Brain by High‐Performance Liquid Chromatography with Electrochemical Detection

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Publisher
Wiley
Copyright
Copyright © 1982 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1982.tb07897.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: A method using reversed‐phase ion‐pair high‐performance liquid chromatography with electrochemical detection for the simultaneous determination of tryptophan (TRP), 3,4‐dihydroxyphenylalanine (DOPA), and their metabolites in whole brain, small‐brain parts, and cerebrospinal fluid of rats has been developed. The sample preparation requires only homogenization in perchloric acid and centrifugation before injection onto the column. With a LiChrosorb RP‐18 (10 μm) column and a mobile phase consisting of a phosphate (NaH2PO4, 0.1 M)‐methanol mixture with octylsulfonate (2.6 × 10−3M) at pH 3.35 and 26°C, the separation of DOPA, dopamine, norepinephrine, 3,4‐dihydroxyphenylacetic acid, homovanillic acid, 4‐hydroxy‐3‐methoxyphenylalanine, TRP, 5‐hydroxytryptophan (SHTP), serotonin, and 5‐hydroxyindole‐acetic acid was achieved. The method has been applied to study the effect of α‐monofluoromethyldopa alone and in combination with L‐DOPA or L‐5‐HTP, on the catechol and 5‐OH indole levels in brain and CSF of the rat.

Journal

Journal of NeurochemistryWiley

Published: May 1, 1982

References

  • Further studies on the inhibition of monoamine synthesis by monofluoromethyldopa
    Bey, Bey; Jung, Jung; Koch‐Weser, Koch‐Weser; Palfreyman, Palfreyman; Sjoerdsma, Sjoerdsma; Wagner, Wagner; Zraika, Zraika
  • High performance liquid‐chromatographic separation and fluorescence measurement of biogenic amines in plasma, urine and tissue
    Davis, Davis; Gehrke, Gehrke; Gehrke, Gehrke; Cunningham, Cunningham; Kuo, Kuo; Gerhardt, Gerhardt; Johnson, Johnson; Williams, Williams
  • Determination of catecholamine in rat brain parts by reverse phase ion‐pair liquid chromatography
    Felice, Felice; Felice, Felice; Kissinger, Kissinger
  • Dopamine conjugate in cerebrospinal fluid
    Tyce, Tyce; Sharpless, Sharpless; Kerr, Kerr; Muenter, Muenter

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