Signal Amplification Strategy Based on TiO 2 ‐Nanotube Layers and Nanobeads Carrying Quantum Dots for Electrochemiluminescent Immunosensors

Signal Amplification Strategy Based on TiO 2 ‐Nanotube Layers and Nanobeads Carrying Quantum... Self‐organized TiO2‐nanotube layers can be used for immunoassay‐type sensing in combination with amplifying CdTe labels in a direct and very sensitive electrochemiluminescent (ECL) configuration. Key properties for this method are the conductivity of the TiO2 nanotubes, and their transparency for light emitted from the CdTe labels at approximately 2.4 eV. To demonstrate the potential of this platform, we constructed a sandwich‐type immunoassay onto the TiO2‐nanotube wall with a layer of (3‐aminopropyl)triethoxysilane as the cross‐linker for antibody immobilization. For the counter part of the sandwich, we created an amplification system consisting of TiO2 nanobeads carrying the secondary antibody and multiple CdTe quantum dots (multiQD). For antigen (IgG) detection, we find that this combination of 3D transparent electrode with multiQD labels allows for an ECL detection limit of 0.05 pg mL−1 and a linearity of the signal in the range of 0.1–108 pg mL−1. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png ChemistryOpen Wiley

Signal Amplification Strategy Based on TiO 2 ‐Nanotube Layers and Nanobeads Carrying Quantum Dots for Electrochemiluminescent Immunosensors

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Publisher
Wiley
Copyright
© 2013. The Authors. ChemistryOpen published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution Non‐Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
ISSN
2191-1363
eISSN
2191-1363
D.O.I.
10.1002/open.201300003
Publisher site
See Article on Publisher Site

Abstract

Self‐organized TiO2‐nanotube layers can be used for immunoassay‐type sensing in combination with amplifying CdTe labels in a direct and very sensitive electrochemiluminescent (ECL) configuration. Key properties for this method are the conductivity of the TiO2 nanotubes, and their transparency for light emitted from the CdTe labels at approximately 2.4 eV. To demonstrate the potential of this platform, we constructed a sandwich‐type immunoassay onto the TiO2‐nanotube wall with a layer of (3‐aminopropyl)triethoxysilane as the cross‐linker for antibody immobilization. For the counter part of the sandwich, we created an amplification system consisting of TiO2 nanobeads carrying the secondary antibody and multiple CdTe quantum dots (multiQD). For antigen (IgG) detection, we find that this combination of 3D transparent electrode with multiQD labels allows for an ECL detection limit of 0.05 pg mL−1 and a linearity of the signal in the range of 0.1–108 pg mL−1.

Journal

ChemistryOpenWiley

Published: Jun 1, 2013

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