Sequence and expression pattern of the Stra7 ( Gbx‐2 ) homeobox‐containing gene induced by retinoic acid in P19 embryonal carcinoma cells

Sequence and expression pattern of the Stra7 ( Gbx‐2 ) homeobox‐containing gene induced by... The cDNA sequence of Stra7, a retinoic acid (RA)‐inducible gene in P19 embryonal carcinoma (EC) cells, was determined. The deduced Stra7 protein contains a homeodomain highly similar to that of the previously described chicken CHox7 gene product, and is highly conserved during evolution, from hemichordates to vertebrates. The mouse Stra7 cDNA corresponds to the full‐length form of the 77 bp homeodomainencoding cDNA fragment which was previously cloned and termed MMoxA or Gbx‐2. Reversetranscriptase‐PCR analysis revealed the presence of Stra7/Gbx‐2 transcripts in the adult brain, spleen, and female genital tract, whereas no expression could be observed in heart, liver, lung, kidney, or testes. In situ hybridization analysis showed a restricted expression pattern of Stra7/Gbx‐2 in the three primitive germ layers during gastrulation. Restricted expression was also detected in the pharyngeal arches. Subsequently, there were specific expression domains in the developing central nervous system, at the midbrain/hindbrain boundary and later in the cerebellum anlage, in certain rhombomeres, in dorsal regions of the spinal cord, and in the developing dorsal thalamus and corpus striatum. © 1995 wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Developmental Dynamics Wiley

Sequence and expression pattern of the Stra7 ( Gbx‐2 ) homeobox‐containing gene induced by retinoic acid in P19 embryonal carcinoma cells

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Publisher
Wiley
Copyright
Copyright © 1995 Wiley‐Liss, Inc.
ISSN
1058-8388
eISSN
1097-0177
DOI
10.1002/aja.1002040404
pmid
8601031
Publisher site
See Article on Publisher Site

Abstract

The cDNA sequence of Stra7, a retinoic acid (RA)‐inducible gene in P19 embryonal carcinoma (EC) cells, was determined. The deduced Stra7 protein contains a homeodomain highly similar to that of the previously described chicken CHox7 gene product, and is highly conserved during evolution, from hemichordates to vertebrates. The mouse Stra7 cDNA corresponds to the full‐length form of the 77 bp homeodomainencoding cDNA fragment which was previously cloned and termed MMoxA or Gbx‐2. Reversetranscriptase‐PCR analysis revealed the presence of Stra7/Gbx‐2 transcripts in the adult brain, spleen, and female genital tract, whereas no expression could be observed in heart, liver, lung, kidney, or testes. In situ hybridization analysis showed a restricted expression pattern of Stra7/Gbx‐2 in the three primitive germ layers during gastrulation. Restricted expression was also detected in the pharyngeal arches. Subsequently, there were specific expression domains in the developing central nervous system, at the midbrain/hindbrain boundary and later in the cerebellum anlage, in certain rhombomeres, in dorsal regions of the spinal cord, and in the developing dorsal thalamus and corpus striatum. © 1995 wiley‐Liss, Inc.

Journal

Developmental DynamicsWiley

Published: Dec 1, 1995

References

  • Efficient cloning of cDNAs of retinoic acid‐responsive genes in P19 embryonal carcinoma cells and characterization of a novel mouse gene, STRA1 (mouse LERK‐2)
    Bouillet, Bouillet; Oulad‐Abdelghni, Oulad‐Abdelghni; Vicaire, Vicaire; Garnier, Garnier; Schuhbaur, Schuhbaur; Dollé, Dollé; Chambon, Chambon

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