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Sensitized fluorescence in polynucleotide‐dye complexes and the problem of energy transfer in polynucleotides

Sensitized fluorescence in polynucleotide‐dye complexes and the problem of energy transfer in... New results on sensitization of fluorescence of Profiuvine, Acridine Orange and Ethidium Bromide complexes wilh double stranded poly A‐poly U upon ultraviolet irradiation are presented. Together with older results on dye DNA complex, they are interpreted to show that base to base transfer plays essentially no role compared to direct base to dye transfer. A lower limit of base to base transfer time (which is at the same time a higher limit for life time of fluorescence) in DNA (2. 10−11 sec.) and poly A‐poly U (2. 10−13 sec.) is obtained which cast some doubt upon the validity of calculations of optical properties using the exciton (strong coupling) approximation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biopolymers Wiley

Sensitized fluorescence in polynucleotide‐dye complexes and the problem of energy transfer in polynucleotides

Biopolymers , Volume 10 (10) – Oct 1, 1971

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References (12)

Publisher
Wiley
Copyright
Copyright © 1971 John Wiley & Sons, Inc.
ISSN
0006-3525
eISSN
1097-0282
DOI
10.1002/bip.360101015
Publisher site
See Article on Publisher Site

Abstract

New results on sensitization of fluorescence of Profiuvine, Acridine Orange and Ethidium Bromide complexes wilh double stranded poly A‐poly U upon ultraviolet irradiation are presented. Together with older results on dye DNA complex, they are interpreted to show that base to base transfer plays essentially no role compared to direct base to dye transfer. A lower limit of base to base transfer time (which is at the same time a higher limit for life time of fluorescence) in DNA (2. 10−11 sec.) and poly A‐poly U (2. 10−13 sec.) is obtained which cast some doubt upon the validity of calculations of optical properties using the exciton (strong coupling) approximation.

Journal

BiopolymersWiley

Published: Oct 1, 1971

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