Sensitivity and specificity of a new automated system for the detection of hepatitis B virus, hepatitis C virus, and human immunodeficiency virus nucleic acid in blood and plasma donations

Sensitivity and specificity of a new automated system for the detection of hepatitis B virus,... ABBREVIATIONSCASCOBAS AmpliScreenICinternal controlCEConformité EuropéeneIDTindividual donation testingLoD(s)limit(s) of detectionMP6minipool of up to 6 donationsMP96minipool of up to 96 donationsMPXv1cobas TaqScreen MPX TestMPXv2cobas TaqScreen MPX Test, version 2.0OBI(s)occult HBV infection(s)RMSRoche Molecular Systems, Inc.RRrepeat reactiveWP(s)window period(s)Multiplex tests for the detection of nucleic acids from hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) are in widespread use for blood and plasma donation screening. These tests supplement serologic testing by detecting early infection before seroconversion, as well as chronic occult HBV infections (OBIs) and have demonstrably improved the safety of the blood supply. Testing is typically accomplished through the use of automated instrumentation, and reagents and instruments are subject to continuous improvement.Current technologies for this testing employ nucleic acid amplification, generally by polymerase chain reaction (PCR) or transcription‐mediated amplification. A relatively recent advance consists of multidye real‐time PCR, which identifies the amplified viral target (HIV, HBV, or HCV) during the primary multiplex screen, eliminating the need for a second round of discriminatory testing.Assays used for testing blood donations require a high level of sensitivity to assure detection of infectious blood units but at the same time must also have high specificity to avoid wastage of safe donations and unnecessary http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Sensitivity and specificity of a new automated system for the detection of hepatitis B virus, hepatitis C virus, and human immunodeficiency virus nucleic acid in blood and plasma donations

Loading next page...
 
/lp/wiley/sensitivity-and-specificity-of-a-new-automated-system-for-the-pbpEBcr28P
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
© 2018 AABB
ISSN
0041-1132
eISSN
1537-2995
D.O.I.
10.1111/trf.14457
Publisher site
See Article on Publisher Site

Abstract

ABBREVIATIONSCASCOBAS AmpliScreenICinternal controlCEConformité EuropéeneIDTindividual donation testingLoD(s)limit(s) of detectionMP6minipool of up to 6 donationsMP96minipool of up to 96 donationsMPXv1cobas TaqScreen MPX TestMPXv2cobas TaqScreen MPX Test, version 2.0OBI(s)occult HBV infection(s)RMSRoche Molecular Systems, Inc.RRrepeat reactiveWP(s)window period(s)Multiplex tests for the detection of nucleic acids from hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) are in widespread use for blood and plasma donation screening. These tests supplement serologic testing by detecting early infection before seroconversion, as well as chronic occult HBV infections (OBIs) and have demonstrably improved the safety of the blood supply. Testing is typically accomplished through the use of automated instrumentation, and reagents and instruments are subject to continuous improvement.Current technologies for this testing employ nucleic acid amplification, generally by polymerase chain reaction (PCR) or transcription‐mediated amplification. A relatively recent advance consists of multidye real‐time PCR, which identifies the amplified viral target (HIV, HBV, or HCV) during the primary multiplex screen, eliminating the need for a second round of discriminatory testing.Assays used for testing blood donations require a high level of sensitivity to assure detection of infectious blood units but at the same time must also have high specificity to avoid wastage of safe donations and unnecessary

Journal

TransfusionWiley

Published: Jan 1, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off