Selective Upregulation of Cytokine Receptor Subchain and Their Intracellular Signalling Molecules After Peripheral Nerve Injury

Selective Upregulation of Cytokine Receptor Subchain and Their Intracellular Signalling Molecules... Numerous studies have suggested that growth factors and cytokines play an important role in the survival of injured neurons and in neurite elongation. Therefore, intracellular signalling pathways activated by growth factors and cytokine receptors play an important role in neuronal survival or for the re‐establishment of connection. Since the JAK (janus kinase)‐STAT (signal transducers and activators of transcription) signal transduction pathway is known to play a major role in cytokine receptor signalling, we first examined regulation of JAK gene expression following peripheral nerve injury by in situ hybridization histochemistry. The rat hypoglossal nerve was axotomized unilaterally and the mRNA levels for JAKI, JAK2. JAK3 and TYK2 were examined in the hypoglossal nucleus at postoperative times ranging from 1 to 35 days. Among the JAK family members, JAK2 and JAK3 were substantially increased in injured hypoglossal motoneurons, whereas no significant increases were observed for JAK1 and TYK2. These changes were further confirmed by immunohistochemistry using antibodies specific to JAKP and JAK3. In addition, we examined the JAK2 and JAK3 associated cytokine receptor components, IL‐2R y and gp130, which are common to various cytokine receptors. Among these, gp130 immunostaining was upregulated after nerve injury. This was also confirmed by in situ hybridization. These results suggest that the injured neuron prepares the molecular machinery involved in certain cytokine receptor signalling pathways at an early phase of the regenerative process, accelerating for the neuron to respond to cytokines that may regulate survival and/or neurite elongation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

Selective Upregulation of Cytokine Receptor Subchain and Their Intracellular Signalling Molecules After Peripheral Nerve Injury

Loading next page...
 
/lp/wiley/selective-upregulation-of-cytokine-receptor-subchain-and-their-gG7ocYZv3v
Publisher
Wiley
Copyright
Copyright © 1997 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
D.O.I.
10.1111/j.1460-9568.1997.tb01455.x
Publisher site
See Article on Publisher Site

Abstract

Numerous studies have suggested that growth factors and cytokines play an important role in the survival of injured neurons and in neurite elongation. Therefore, intracellular signalling pathways activated by growth factors and cytokine receptors play an important role in neuronal survival or for the re‐establishment of connection. Since the JAK (janus kinase)‐STAT (signal transducers and activators of transcription) signal transduction pathway is known to play a major role in cytokine receptor signalling, we first examined regulation of JAK gene expression following peripheral nerve injury by in situ hybridization histochemistry. The rat hypoglossal nerve was axotomized unilaterally and the mRNA levels for JAKI, JAK2. JAK3 and TYK2 were examined in the hypoglossal nucleus at postoperative times ranging from 1 to 35 days. Among the JAK family members, JAK2 and JAK3 were substantially increased in injured hypoglossal motoneurons, whereas no significant increases were observed for JAK1 and TYK2. These changes were further confirmed by immunohistochemistry using antibodies specific to JAKP and JAK3. In addition, we examined the JAK2 and JAK3 associated cytokine receptor components, IL‐2R y and gp130, which are common to various cytokine receptors. Among these, gp130 immunostaining was upregulated after nerve injury. This was also confirmed by in situ hybridization. These results suggest that the injured neuron prepares the molecular machinery involved in certain cytokine receptor signalling pathways at an early phase of the regenerative process, accelerating for the neuron to respond to cytokines that may regulate survival and/or neurite elongation.

Journal

European Journal of NeuroscienceWiley

Published: May 1, 1997

References

  • Accelerated nerve regeneration in mice by upregulated expression of IL‐6 and IL‐6 receptor after trauma
    Hirota, Hirota; Kiyama, Kiyama; Kishimoto, Kishimoto; Taga, Taga
  • Signaling through the hematopoietic cytokine receptors
    Ihle, Ihle; Witthuhn, Witthuhn; Quelle, Quelle; Yamamoto, Yamamoto; Silvennoinen, Silvennoinen
  • Interleukin‐6 production by brain tissue and cultured astrocytes infected with Theiler's murine encephalomyelitis virus
    Rubio, Rubio; Sierra, Sierra
  • Cellular localization of interleukin 6 mRNA and interleukin 6 receptor mRNA in rat brain
    Schobitz, Schobitz; De, De; Sutanto, Sutanto; Holsboer, Holsboer

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off