Scythe: a novel reaper‐binding apoptotic regulator

Scythe: a novel reaper‐binding apoptotic regulator Reaper is a central regulator of apoptosis in Drosophila melanogaster. With no obvious catalytic activity or homology to other known apoptotic regulators, reaper's mechanism of action has been obscure. We recently reported that recombinant Drosophila reaper protein induced rapid mitochondrial cytochrome c release, caspase activation and apoptotic nuclear fragmentation in extracts of Xenopus eggs. We now report the purification of a 150 kDa reaper‐interacting protein from Xenopus egg extracts, which we have named Scythe. Scythe is highly conserved among vertebrates and contains a ubiquitin‐like domain near its N‐terminus. Immunodepletion of Scythe from extracts completely prevented reaper‐induced apoptosis without affecting apoptosis triggered by activated caspases. Moreover, a truncated variant of Scythe lacking the N‐terminal domain induced apoptosis even in the absence of reaper. These data suggest that Scythe is a novel apoptotic regulator that is an essential component in the pathway of reaper‐induced apoptosis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

Scythe: a novel reaper‐binding apoptotic regulator

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Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc
ISSN
0261-4189
eISSN
1460-2075
DOI
10.1093/emboj/17.21.6135
Publisher site
See Article on Publisher Site

Abstract

Reaper is a central regulator of apoptosis in Drosophila melanogaster. With no obvious catalytic activity or homology to other known apoptotic regulators, reaper's mechanism of action has been obscure. We recently reported that recombinant Drosophila reaper protein induced rapid mitochondrial cytochrome c release, caspase activation and apoptotic nuclear fragmentation in extracts of Xenopus eggs. We now report the purification of a 150 kDa reaper‐interacting protein from Xenopus egg extracts, which we have named Scythe. Scythe is highly conserved among vertebrates and contains a ubiquitin‐like domain near its N‐terminus. Immunodepletion of Scythe from extracts completely prevented reaper‐induced apoptosis without affecting apoptosis triggered by activated caspases. Moreover, a truncated variant of Scythe lacking the N‐terminal domain induced apoptosis even in the absence of reaper. These data suggest that Scythe is a novel apoptotic regulator that is an essential component in the pathway of reaper‐induced apoptosis.

Journal

The EMBO JournalWiley

Published: Feb 2, 1998

Keywords: ; ; ;

References

  • The cell‐death machine
    Chinnaiyan, A; Dixit, VM
  • Mechanisms and functions of cell death
    Ellis, RE; Yuan, J; Horvitz, HR
  • Reaper‐induced apoptosis in a vertebrate system
    Evans, EK; Kuwana, T; Strum, SL; Smith, JJ; Newmeyer, DD; Kornbluth, S
  • Cytochrome c activation of CPP32‐like proteolysis plays a critical role in a Xenopus cell‐free apoptosis system
    Kluck, RM; Martin, SJ; Hoffman, BM; Zhou, JS; Green, DR; Newmeyer, DD
  • Bcl‐2 and the regulation of programmed cell death
    Reed, JC
  • Cell killing by the Drosophila gene reaper
    White, K; Tahaoglu, E; Steller, H

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