Screening techniques for detecting allelic variation in DNA sequences

Screening techniques for detecting allelic variation in DNA sequences This article reviews four ‘DNA screening techniques’, namely heteroduplex analysis, single‐strand conformational polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) as tools for the study of allelic variation in natural populations. The resolving power, advantages, and limitations of each technique are discussed and compared. We also provide some criteria for choosing among techniques and illustrate some practical issues with examples taken primarily from our own laboratory experience. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Ecology Wiley

Screening techniques for detecting allelic variation in DNA sequences

Molecular Ecology, Volume 2 (2) – Apr 1, 1993

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Publisher
Wiley
Copyright
"Copyright © 1993 Wiley Subscription Services, Inc., A Wiley Company"
ISSN
0962-1083
eISSN
1365-294X
D.O.I.
10.1111/j.1365-294X.1993.tb00006.x
Publisher site
See Article on Publisher Site

Abstract

This article reviews four ‘DNA screening techniques’, namely heteroduplex analysis, single‐strand conformational polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) as tools for the study of allelic variation in natural populations. The resolving power, advantages, and limitations of each technique are discussed and compared. We also provide some criteria for choosing among techniques and illustrate some practical issues with examples taken primarily from our own laboratory experience.

Journal

Molecular EcologyWiley

Published: Apr 1, 1993

Keywords: ; ; ; ; ;

References

  • Genes in Ecology
    Burke, TJ; Rainey, WE; White, TJ
  • PCR‐SSCP: a simple and sensitive method for detection of mutations in the genomic DNA
    Hayashi, K.

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