Screening blood donations for viral genomes: multicenter study of real‐ time simulation using pooled samples on the model of hepatitis C virus RNA detection

Screening blood donations for viral genomes: multicenter study of real‐ time simulation using... BACKGROUND: The systematic screening for several blood‐borne viral genomes in blood donations is a complementary safety measure planned or discussed by the national authorities of several countries. STUDY DESIGN AND METHODS: To appreciate the feasibility of such screening using pooled samples, a multicenter study of real‐time simulation has been performed on the model of hepatitis C virus (HCV) infection. Four blood transfusion center laboratories and two research and diagnosis laboratories simultaneously screened, by several HCV RNA polymerase chain reaction assays, a panel of plasma sample pools of different sizes (500, 100, and 10 samples), collected from HCV‐infected or HCV‐ uninfected blood donors. One viremic plasma was introduced in each pool. HCV RNA was detected by in‐house polymerase chain reaction procedures or by standardized manual or semi‐automated polymerase chain reaction assays. RESULTS: The results indicate the feasibility of sample pooling, which renders the screening for viral genomes by molecular biology techniques applicable in the short term and the importance of the experience of laboratory personnel in the use of molecular biology tools. CONCLUSION: The improvement of standardized assays needs to be continued, and training of laboratory staff members appears to be a crucial step before systematic screening of blood donations for viral genomes by molecular biology techniques can occur. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Screening blood donations for viral genomes: multicenter study of real‐ time simulation using pooled samples on the model of hepatitis C virus RNA detection

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Publisher
Wiley
Copyright
1998 AABB
ISSN
0041-1132
eISSN
1537-2995
DOI
10.1046/j.1537-2995.1998.381098440855.x
Publisher site
See Article on Publisher Site

Abstract

BACKGROUND: The systematic screening for several blood‐borne viral genomes in blood donations is a complementary safety measure planned or discussed by the national authorities of several countries. STUDY DESIGN AND METHODS: To appreciate the feasibility of such screening using pooled samples, a multicenter study of real‐time simulation has been performed on the model of hepatitis C virus (HCV) infection. Four blood transfusion center laboratories and two research and diagnosis laboratories simultaneously screened, by several HCV RNA polymerase chain reaction assays, a panel of plasma sample pools of different sizes (500, 100, and 10 samples), collected from HCV‐infected or HCV‐ uninfected blood donors. One viremic plasma was introduced in each pool. HCV RNA was detected by in‐house polymerase chain reaction procedures or by standardized manual or semi‐automated polymerase chain reaction assays. RESULTS: The results indicate the feasibility of sample pooling, which renders the screening for viral genomes by molecular biology techniques applicable in the short term and the importance of the experience of laboratory personnel in the use of molecular biology tools. CONCLUSION: The improvement of standardized assays needs to be continued, and training of laboratory staff members appears to be a crucial step before systematic screening of blood donations for viral genomes by molecular biology techniques can occur.

Journal

TransfusionWiley

Published: Oct 1, 1998

References

  • International collaborative study on the second Eurohep HCV‐RNA reference panel
    Damen, Damen; Cuypers, Cuypers; Zaaijer, Zaaijer
  • What technique should be used for routine detection and quantification of HBV DNA in clinical samples
    Pawlotsky, Pawlotsky; Bastie, Bastie; Lonjon, Lonjon

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