Salmon and Trout Analysis by PCR‐RFLP for Identity Authentication

Salmon and Trout Analysis by PCR‐RFLP for Identity Authentication ABSTRACT Raw and smoked Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) were differentiated by PCR amplification of a 950 bp conserved fragment of the mitochondrial 16S rRNA gene followed by restriction site analysis with the endonucleases Hpa I and Bst Ell. Salmon PCR products digested with Hpa I yielded two fragments of 804 bp and 146 bp, while trout PCR products were not cleaved by this enzyme. However, Bst Ell did not cleave salmon PCR products while two bands of 513 bp and 437 bp were produced when trout samples were cleaved with this enzyme. This genetic marker could be very useful for detecting fraudulent substitution of lower valued smoked trout. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Food Science Wiley

Salmon and Trout Analysis by PCR‐RFLP for Identity Authentication

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Publisher
Wiley
Copyright
Copyright © 1999 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-1147
eISSN
1750-3841
DOI
10.1111/j.1365-2621.1999.tb15053.x
Publisher site
See Article on Publisher Site

Abstract

ABSTRACT Raw and smoked Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) were differentiated by PCR amplification of a 950 bp conserved fragment of the mitochondrial 16S rRNA gene followed by restriction site analysis with the endonucleases Hpa I and Bst Ell. Salmon PCR products digested with Hpa I yielded two fragments of 804 bp and 146 bp, while trout PCR products were not cleaved by this enzyme. However, Bst Ell did not cleave salmon PCR products while two bands of 513 bp and 437 bp were produced when trout samples were cleaved with this enzyme. This genetic marker could be very useful for detecting fraudulent substitution of lower valued smoked trout.

Journal

Journal of Food ScienceWiley

Published: May 1, 1999

References

  • Identification of flatfish species using polymerase chain reaction (PCR) amplification and restriction analysis of the cytochrome b gene
    Céspedes, Céspedes; García, García; Carrera, Carrera; González, González; Sanz, Sanz; Hernández, Hernández; Martín, Martín
  • Capillary zone electrophoresis of fish muscle sarcoplasmic proteins
    LeBlanc, LeBlanc; LeBlanc, LeBlanc
  • Evolution of animal mitochondrial DNA: relevance for population biology and systematics
    Moritz, Moritz; Dowling, Dowling; Brown, Brown
  • Application of PCR‐SSCP to species identification of fishery products
    Rehbein, Rehbein; Kress, Kress; Schmidt, Schmidt

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