An approximate analytical method is described for the rapid routine determination of carbohydrates and lignin in herbage. Dried milled samples are extracted successively with diethyl ether, 0.5% ammonium oxalate solution, N‐H2SO4, and 72% H2SO4. Soluble carbohydrates in the ammonium oxalate extract are determined with anthrone, and hydrolysed pentosans and hexosans in the combined N‐H2SO4 and 72% H2SO4 extracts are determined with aniline acetate and chromotropic acid respectively. The method gives a maximum variation of 4% from the mean of duplicates with each carbohydrate component. The residual material is ashed to obtain crude lignin, and a nitrogen correction applied.
Journal of the Science of Food and Agriculture – Wiley
Published: Feb 1, 1961
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