Role of the plasmin‐generating system in the developing nervous tissue: I. Proteolysis as a mitogenic signal for the glial cells

Role of the plasmin‐generating system in the developing nervous tissue: I. Proteolysis as a... The role of the plasmin‐generating system, a serum component, in the development of dissociated embryonic chick spinal cord cells in culture was studied. Studies were performed in a defined system where the cells were maintained in a serum‐free medium. Under these conditions the cells produce plasminogen activator. It was found that plasminogen, when added to the chemically defined culture medium at concentrations of 0.2–0.75 μg/ml, stimulates (3H)thymidine uptake (as expressed per total DNA) in a dose‐response manner. This mitogenic effect is abolished by the protease inhibitors leupeptin and aprotinin. Trypsin, but not chymotrypsin, can produce similar effects. It is concluded that plasmin, which is produced as a result of the activation of plasminogen, is a component that serves as a proliferation factor in developing spinal cords in culture. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

Role of the plasmin‐generating system in the developing nervous tissue: I. Proteolysis as a mitogenic signal for the glial cells

Journal of Neuroscience Research, Volume 8 (2‐3) – Jan 1, 1982

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Publisher
Wiley
Copyright
Copyright © 1982 Alan R. Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
D.O.I.
10.1002/jnr.490080237
Publisher site
See Article on Publisher Site

Abstract

The role of the plasmin‐generating system, a serum component, in the development of dissociated embryonic chick spinal cord cells in culture was studied. Studies were performed in a defined system where the cells were maintained in a serum‐free medium. Under these conditions the cells produce plasminogen activator. It was found that plasminogen, when added to the chemically defined culture medium at concentrations of 0.2–0.75 μg/ml, stimulates (3H)thymidine uptake (as expressed per total DNA) in a dose‐response manner. This mitogenic effect is abolished by the protease inhibitors leupeptin and aprotinin. Trypsin, but not chymotrypsin, can produce similar effects. It is concluded that plasmin, which is produced as a result of the activation of plasminogen, is a component that serves as a proliferation factor in developing spinal cords in culture.

Journal

Journal of Neuroscience ResearchWiley

Published: Jan 1, 1982

References

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