IntroductionThe development of next‐generation sequencing technologies (NGS) has opened new opportunities to better characterize complex eukaryotic cells at the transcriptome level. RNA sequencing (RNA‐Seq) has proven highly accurate and reproducible on expression level measurements and has many advantages over previous technologies such as microarrays. Almost the whole transcriptome can be measured at once and it is not limited to organisms with known genomic sequence. RNA‐Seq provides single‐base resolution for annotation, distinguishing different splice variant forms and single‐nucleotide polymorphisms. It has very low background signal, enabling measurement of low abundant transcripts, and a wide dynamic range as there is no upper‐limit for quantification. Finally, a relatively small amount of total RNA is required compared to microarrays.The comparison of different cell scenarios is a way to extract meaningful information out of “omics” approaches. Thereby, contrasting transcriptomics data from different culture conditions or cell lines can allow finding features related to those specific phenotypes. Chinese Hamster Ovary (CHO) cells are the preferred host for recombinant therapeutic protein production, currently being used for the production of five of the top ten blockbuster drugs. The recombinant protein yield in mammalian cells has increased more than 100‐fold over the past three decades largely through media
Biotechnology Journal – Wiley
Published: Jan 1, 2018
Keywords: ; ; ; ;
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