Retinoids regulate the anterior expression boundaries of 5′ Hoxb genes in posterior hindbrain

Retinoids regulate the anterior expression boundaries of 5′ Hoxb genes in posterior hindbrain We describe the regulatory interactions that cause anterior extension of the mouse 5′ Hoxb expression domains from spinal cord levels to their definitive boundaries in the posterior hindbrain between embryonic day E10 and E11.5. This anterior expansion is retinoid dependent since it does not occur in mouse embryos deficient for the retinoic acid‐synthesizing enzyme retinaldehyde dehydrogenase 2. A retinoic acid response element (RARE) was identified downstream of Hoxb5 and shown to be essential for expression of Hoxb5 and Hoxb8 reporter transgenes in the anterior neural tube. The spatio‐temporal activity of this element overlaps with rostral extension of the expression domain of endogenous Hoxb5, Hoxb6 and Hoxb8 into the posterior hindbrain. The RARE and surrounding sequences are found at homologous positions in the human, mouse and zebrafish genome, which supports an evolutionarily conserved regulatory function. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

Retinoids regulate the anterior expression boundaries of 5′ Hoxb genes in posterior hindbrain

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Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc
ISSN
0261-4189
eISSN
1460-2075
D.O.I.
10.1093/emboj/cdg029
Publisher site
See Article on Publisher Site

Abstract

We describe the regulatory interactions that cause anterior extension of the mouse 5′ Hoxb expression domains from spinal cord levels to their definitive boundaries in the posterior hindbrain between embryonic day E10 and E11.5. This anterior expansion is retinoid dependent since it does not occur in mouse embryos deficient for the retinoic acid‐synthesizing enzyme retinaldehyde dehydrogenase 2. A retinoic acid response element (RARE) was identified downstream of Hoxb5 and shown to be essential for expression of Hoxb5 and Hoxb8 reporter transgenes in the anterior neural tube. The spatio‐temporal activity of this element overlaps with rostral extension of the expression domain of endogenous Hoxb5, Hoxb6 and Hoxb8 into the posterior hindbrain. The RARE and surrounding sequences are found at homologous positions in the human, mouse and zebrafish genome, which supports an evolutionarily conserved regulatory function.

Journal

The EMBO JournalWiley

Published: Jan 15, 2003

References

  • Regulation of the Hoxb‐8 gene: synergism between multimerized cis ‐acting elements increases responsiveness to positional information
    Charité, Charité; de Graaff, de Graaff; Vogels, Vogels; Meijlink, Meijlink; Deschamps, Deschamps
  • Analysis of lacZ reporter genes in transgenic embryos suggests the presence of several cis ‐acting regulatory elements in the murine Hoxb‐6 gene
    Eid, Eid; Koseki, Koseki; Schughart, Schughart
  • Retinoid signalling and hindbrain patterning
    Gavalas, Gavalas; Krumlauf, Krumlauf
  • Selectivity, sharing and competitive interactions in the regulation of Hoxb genes
    Sharpe, Sharpe; Nonchev, Nonchev; Gould, Gould; Whiting, Whiting; Krumlauf, Krumlauf
  • Complementary domains of retinoic acid production and degradation in the early chick embryo
    Swindell, Swindell; Thaller, Thaller; Sockanathan, Sockanathan; Petkovitch, Petkovitch; Jessel, Jessel; Eichele, Eichele
  • Deletion of a HoxD enhancer induces transcriptional heterochrony leading to transposition of the sacrum
    Zákány, Zákány; Gerard, Gerard; Favier, Favier; Duboule, Duboule

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