Restriction fragment length polymorphism among Israeli Holstein‐Friesian dairy bulls*

Restriction fragment length polymorphism among Israeli Holstein‐Friesian dairy bulls* Summary Israeli Holstein‐Friesian dairy bulls were screened for restriction fragment length polymorphisms by hybridizing cloned DNA probes for bovine growth hormone, for chymosin, and for rat muscle beta‐actin to restriction endonuclease‐digested DNA immobilized on nitrocellulose filters. The population proved to be polymorphic at the growth hormone locus, with evidence consistent with the phenotypes being inherited in allelic fashion. A low level of polymorphism was also observed at one of the beta‐actin gene family loci. The chymosin locus was monomorphic with the restriction enzymes utilized. The results illustrate the power of restriction fragment length polymorphism methodology in visualizing genetic variability in dairy cattle populations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Animal Genetics Wiley

Restriction fragment length polymorphism among Israeli Holstein‐Friesian dairy bulls*

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Publisher
Wiley
Copyright
Copyright © 1986 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0268-9146
eISSN
1365-2052
DOI
10.1111/j.1365-2052.1986.tb03185.x
Publisher site
See Article on Publisher Site

Abstract

Summary Israeli Holstein‐Friesian dairy bulls were screened for restriction fragment length polymorphisms by hybridizing cloned DNA probes for bovine growth hormone, for chymosin, and for rat muscle beta‐actin to restriction endonuclease‐digested DNA immobilized on nitrocellulose filters. The population proved to be polymorphic at the growth hormone locus, with evidence consistent with the phenotypes being inherited in allelic fashion. A low level of polymorphism was also observed at one of the beta‐actin gene family loci. The chymosin locus was monomorphic with the restriction enzymes utilized. The results illustrate the power of restriction fragment length polymorphism methodology in visualizing genetic variability in dairy cattle populations.

Journal

Animal GeneticsWiley

Published: Feb 1, 1986

Keywords: ; ; ; ;

References

  • Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping, and costs
    Beckmann, J.S.; Soller, M.

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