Response to ‘The evolving mystery of why skeletal muscle is spared in seropositive neuromyelitis optica’

Response to ‘The evolving mystery of why skeletal muscle is spared in seropositive... Dear Editor,We would like to thank you for the opportunity to respond to the questions raised in Dr Verkman's letter and to elucidate related aspects. We also thank Dr Verkman and colleagues for their attention to our study.The use of g‐STED super‐resolution microscopy versus freeze‐fracture electron microscopy (FFEM) to analyse skeletal muscle and brain AQP4 supramolecular assemblies (OAPs) used in our study has been disputed by Verkman et al. While we agree that FFEM is the gold standard to visualise OAPs and also measure their size, we also are aware that the very small amount of the plasma membrane that can be suitable for analysis represents a major limit to obtaining statistically significant data (such as the OAP dimension) representative of the entire tissue. In contrast, STED microscopy has the enormous advantage of analysing, in real time, very large portions of the plasma membrane, with a resolution that in our setup can reach approximately 30 nm, providing the possibility to have a more complete vision of the entire tissue and handle a large amount of data.Considering the ‘contradiction of available data’, Verkman et al. refer to an FFEM study on OAP structure and organisation performed before the identification of http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular and Molecular Medicine Wiley

Response to ‘The evolving mystery of why skeletal muscle is spared in seropositive neuromyelitis optica’

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine
ISSN
1582-1838
eISSN
1582-4934
D.O.I.
10.1111/jcmm.13513
Publisher site
See Article on Publisher Site

Abstract

Dear Editor,We would like to thank you for the opportunity to respond to the questions raised in Dr Verkman's letter and to elucidate related aspects. We also thank Dr Verkman and colleagues for their attention to our study.The use of g‐STED super‐resolution microscopy versus freeze‐fracture electron microscopy (FFEM) to analyse skeletal muscle and brain AQP4 supramolecular assemblies (OAPs) used in our study has been disputed by Verkman et al. While we agree that FFEM is the gold standard to visualise OAPs and also measure their size, we also are aware that the very small amount of the plasma membrane that can be suitable for analysis represents a major limit to obtaining statistically significant data (such as the OAP dimension) representative of the entire tissue. In contrast, STED microscopy has the enormous advantage of analysing, in real time, very large portions of the plasma membrane, with a resolution that in our setup can reach approximately 30 nm, providing the possibility to have a more complete vision of the entire tissue and handle a large amount of data.Considering the ‘contradiction of available data’, Verkman et al. refer to an FFEM study on OAP structure and organisation performed before the identification of

Journal

Journal of Cellular and Molecular MedicineWiley

Published: Jan 1, 2018

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