RESPIRATION IN VITRO OF SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEX

RESPIRATION IN VITRO OF SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEX Abstract— —(1) The respiratory properties of synaptosomes and mitochondria from mammalian cerebral cortex are compared. (2) Synaptosome showed high and linear respiration with glucose and pyruvate as substrates in Krebs‐Ringer media. Mitochondria showed such respiration only with pyruvate as substrate in media lacking Na and high in K and phosphate. (3) Exposure of synaptosomes to hypotonic media caused loss of lactate dehydrogenase (LDH) and protein, and respiration diminished and became non‐linear. (4) Both ATP and phosphocreatine were synthesised by synaptosomes with glucose as substrate. ATP was synthesised by mitochondria in the presence of pyruvate. (5) Synaptosome but not mitochondria showed some capacity for active accumulation of potassium. (6) Both mitochondria and synaptosomes respired with glutamate as substrate. Glutamate caused 80 per cent loss of ATP and phosphocreatine in synaptosomes but did not diminish the level of mitochondrial ATP. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

RESPIRATION IN VITRO OF SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEX

Journal of Neurochemistry, Volume 16 (5) – May 1, 1969

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Publisher
Wiley
Copyright
Copyright © 1969 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1969.tb06444.x
Publisher site
See Article on Publisher Site

Abstract

Abstract— —(1) The respiratory properties of synaptosomes and mitochondria from mammalian cerebral cortex are compared. (2) Synaptosome showed high and linear respiration with glucose and pyruvate as substrates in Krebs‐Ringer media. Mitochondria showed such respiration only with pyruvate as substrate in media lacking Na and high in K and phosphate. (3) Exposure of synaptosomes to hypotonic media caused loss of lactate dehydrogenase (LDH) and protein, and respiration diminished and became non‐linear. (4) Both ATP and phosphocreatine were synthesised by synaptosomes with glucose as substrate. ATP was synthesised by mitochondria in the presence of pyruvate. (5) Synaptosome but not mitochondria showed some capacity for active accumulation of potassium. (6) Both mitochondria and synaptosomes respired with glutamate as substrate. Glutamate caused 80 per cent loss of ATP and phosphocreatine in synaptosomes but did not diminish the level of mitochondrial ATP.

Journal

Journal of NeurochemistryWiley

Published: May 1, 1969

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