Repeatability of 2 Methods for Assessment of Insulin Sensitivity and Glucose Dynamics in Horses

Repeatability of 2 Methods for Assessment of Insulin Sensitivity and Glucose Dynamics in Horses Both the euglycemic‐hyperinsulinemic clamp (EHC) and minimal model analysis of the frequently sampled intravenous glucose tolerance test (FSIGT) have been applied for measurement of insulin sensitivity in horses. However, no published data are available on the reproducibility of these methods. Therefore, the objective of this study was to evaluate the variation and repeatability of measures of glucose dynamics and insulin sensitivity in horses derived from minimal model analysis of the FSIGT and from the EHC method. Six healthy horses underwent both the FSIGT and EHC on 2 occasions over a 4‐week period, with a minimum of 5 days between tests. Coefficient of variation (CV) and intraclass correlation coefficient (ICC) were calculated for measures of glucose metabolism and insulin sensitivity derived from each test. In the EHC, insulin sensitivity, expressed as the amount of metabolized glucose (M) per unit of serum insulin (I) (M/I ratio), averaged 0.19 ± 0.06 ± 10‐4 mmol/kg/min‐(pmol/L)‐1 with an average interday CV of 14.1 ± 5.7% (range, 7–20%) and ICC of 0.74. Minimal model analysis of the FSIGT demonstrated mean insulin sensitivity (Si) of 0.49 ± 0.17 × 10‐4/min × (pmol/L)‐1 with an average interday CV of 23.7 ± 11.2% (range, 9–35%) and ICC of 0.33. Mean CV and ICC for minimal model glucose effectiveness (Sg) and acute insulin response (AIRg) were, respectively, 26.4 ± 11.2% (range 13–40%) and 0.10 and 11.7 ± 6.5% (range 7–21%) and 0.98. Insulin sensitivity measured by the EHC has lower interday variation when compared with the minimal model estimate derived from the FSIGT. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Veterinary Internal Medicine Wiley

Repeatability of 2 Methods for Assessment of Insulin Sensitivity and Glucose Dynamics in Horses

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Publisher
Wiley
Copyright
© 2005 American College of Veterinary Internal Medicine
ISSN
0891-6640
eISSN
1939-1676
D.O.I.
10.1111/j.1939-1676.2005.tb02782.x
Publisher site
See Article on Publisher Site

Abstract

Both the euglycemic‐hyperinsulinemic clamp (EHC) and minimal model analysis of the frequently sampled intravenous glucose tolerance test (FSIGT) have been applied for measurement of insulin sensitivity in horses. However, no published data are available on the reproducibility of these methods. Therefore, the objective of this study was to evaluate the variation and repeatability of measures of glucose dynamics and insulin sensitivity in horses derived from minimal model analysis of the FSIGT and from the EHC method. Six healthy horses underwent both the FSIGT and EHC on 2 occasions over a 4‐week period, with a minimum of 5 days between tests. Coefficient of variation (CV) and intraclass correlation coefficient (ICC) were calculated for measures of glucose metabolism and insulin sensitivity derived from each test. In the EHC, insulin sensitivity, expressed as the amount of metabolized glucose (M) per unit of serum insulin (I) (M/I ratio), averaged 0.19 ± 0.06 ± 10‐4 mmol/kg/min‐(pmol/L)‐1 with an average interday CV of 14.1 ± 5.7% (range, 7–20%) and ICC of 0.74. Minimal model analysis of the FSIGT demonstrated mean insulin sensitivity (Si) of 0.49 ± 0.17 × 10‐4/min × (pmol/L)‐1 with an average interday CV of 23.7 ± 11.2% (range, 9–35%) and ICC of 0.33. Mean CV and ICC for minimal model glucose effectiveness (Sg) and acute insulin response (AIRg) were, respectively, 26.4 ± 11.2% (range 13–40%) and 0.10 and 11.7 ± 6.5% (range 7–21%) and 0.98. Insulin sensitivity measured by the EHC has lower interday variation when compared with the minimal model estimate derived from the FSIGT.

Journal

Journal of Veterinary Internal MedicineWiley

Published: Nov 1, 2005

References

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