Remodeling of dermal collagen in photoaged skin using low‐dose 5‐aminolevulinic acid photodynamic therapy occurs via the transforming growth factor‐β pathway

Remodeling of dermal collagen in photoaged skin using low‐dose 5‐aminolevulinic acid... 5‐Aminolevulinic acid photodynamic therapy (ALA‐PDT) is known to be effective in the treatment of photoaged skin. However, the molecular mechanisms still remain elusive. Protoporphyrin IX (PpIX) fluorescence is primarily located in the epidermis while ALA‐PDT affects the dermal collagen, presumably by an indirect mechanism. This study aimed to investigate the molecular communication in low‐dose ALA‐PDT occurring between epidermal keratinocytes and dermal fibroblasts. Western blotting and enzyme‐linked immunosorbent assays were performed to evaluate collagen expression and transforming growth factor‐β (TGF‐β) signaling in human keratinocytes and dermal fibroblasts. The impact on fibroblast proliferation was assessed by morphology and proliferating cell nuclear antigen immunofluorescence. Skin biopsies from mice were used to analyze the histological changes in dermal collagen and PpIX distribution. When fibroblasts were cocultured with keratinocytes treated with low‐dose ALA‐PDT, collagen synthesis and fibroblast proliferation were enhanced. Low‐dose ALA‐PDT stimulated TGF‐β1 expression in keratinocytes. Fibroblasts cocultured with low‐dose ALA‐PDT‐treated keratinocytes also showed activation of the TGF‐β pathway. In vivo, PpIX fluorescence was densely distributed in photoaged mouse epidermis while collagen in the mouse dermis underwent remodeling. This study suggests that low‐dose ALA‐PDT can stimulate keratinocytes to release TGF‐β1, activating the TGF‐β pathway in dermal fibroblasts to remodel collagen in the dermis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biophotonics Wiley

Remodeling of dermal collagen in photoaged skin using low‐dose 5‐aminolevulinic acid photodynamic therapy occurs via the transforming growth factor‐β pathway

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Publisher
Wiley
Copyright
© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
1864-063X
eISSN
1864-0648
D.O.I.
10.1002/jbio.201700357
Publisher site
See Article on Publisher Site

Abstract

5‐Aminolevulinic acid photodynamic therapy (ALA‐PDT) is known to be effective in the treatment of photoaged skin. However, the molecular mechanisms still remain elusive. Protoporphyrin IX (PpIX) fluorescence is primarily located in the epidermis while ALA‐PDT affects the dermal collagen, presumably by an indirect mechanism. This study aimed to investigate the molecular communication in low‐dose ALA‐PDT occurring between epidermal keratinocytes and dermal fibroblasts. Western blotting and enzyme‐linked immunosorbent assays were performed to evaluate collagen expression and transforming growth factor‐β (TGF‐β) signaling in human keratinocytes and dermal fibroblasts. The impact on fibroblast proliferation was assessed by morphology and proliferating cell nuclear antigen immunofluorescence. Skin biopsies from mice were used to analyze the histological changes in dermal collagen and PpIX distribution. When fibroblasts were cocultured with keratinocytes treated with low‐dose ALA‐PDT, collagen synthesis and fibroblast proliferation were enhanced. Low‐dose ALA‐PDT stimulated TGF‐β1 expression in keratinocytes. Fibroblasts cocultured with low‐dose ALA‐PDT‐treated keratinocytes also showed activation of the TGF‐β pathway. In vivo, PpIX fluorescence was densely distributed in photoaged mouse epidermis while collagen in the mouse dermis underwent remodeling. This study suggests that low‐dose ALA‐PDT can stimulate keratinocytes to release TGF‐β1, activating the TGF‐β pathway in dermal fibroblasts to remodel collagen in the dermis.

Journal

Journal of BiophotonicsWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

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