Regulation of expression of liver‐specific enzymes: II. Activation and chromosomal localization of soluble glutamate‐pyruvate transaminase

Regulation of expression of liver‐specific enzymes: II. Activation and chromosomal localization... SUMMARY 1. The expression of soluble GPT (E.C. 2. 6. 1. 2) was analysed in seventeen independent rat hepatoma/human somatic cell hybrids and in forty‐one subclones derived from two of these hybrids. 2. As judged by electrophoretic mobility, twelve hybrid clones expressed rat GPT activity only and three expressed strong rat and weak human GPT activity but no heteromeric isozymes. In the remaining two hybrids, only human GPT was demonstrable. 3. The segregation of GPT and marker enzymes in the primary hybrid cells and the subclones suggests that the human structural GPT locus is on chromosome 8. 4. The re‐expression of rat GPT in segregating subclones derived from two primary hybrids which had extinguished this function, could not be correlated with presence or absence of any particular human chromosome(s). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annals of Human Genetics Wiley

Regulation of expression of liver‐specific enzymes: II. Activation and chromosomal localization of soluble glutamate‐pyruvate transaminase

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Publisher
Wiley
Copyright
Copyright © 1982 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0003-4800
eISSN
1469-1809
DOI
10.1111/j.1469-1809.1982.tb00704.x
Publisher site
See Article on Publisher Site

Abstract

SUMMARY 1. The expression of soluble GPT (E.C. 2. 6. 1. 2) was analysed in seventeen independent rat hepatoma/human somatic cell hybrids and in forty‐one subclones derived from two of these hybrids. 2. As judged by electrophoretic mobility, twelve hybrid clones expressed rat GPT activity only and three expressed strong rat and weak human GPT activity but no heteromeric isozymes. In the remaining two hybrids, only human GPT was demonstrable. 3. The segregation of GPT and marker enzymes in the primary hybrid cells and the subclones suggests that the human structural GPT locus is on chromosome 8. 4. The re‐expression of rat GPT in segregating subclones derived from two primary hybrids which had extinguished this function, could not be correlated with presence or absence of any particular human chromosome(s).

Journal

Annals of Human GeneticsWiley

Published: May 1, 1982

References

  • Confirmation and further regional assignment of aminoacylase ( ACY‐1 ) on human chromosome 3 using a simplified detection method.
    Voss, Voss; Lerer, Lerer; Povey, Povey; Solomon, Solomon; Bobrow, Bobrow

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