Abstract— The high affinity transport of choline (Ch) and the synthesis of acetylcholine (ACh) were measured in synaptosomes by measuring the utilization of (2H4)Ch. The synthesis of ACh was reduced under several conditions which reduce the availability of acetyl coenzyme A (AcCoA) including no glucose added, replacement of glucose with succinate or impairment of glucose utilization by bromopyr‐uvate, NaCN, or pentobarbital. These conditions did not reduce the amount of unacetylated (2H4)Ch in the synaptosomes indicating that the high affinity transport of Ch is not directly coupled to the synthesis of ACh.
Journal of Neurochemistry – Wiley
Published: May 1, 1978
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