Refrigerated Storage of Washed Red Cells

Refrigerated Storage of Washed Red Cells Abstract. Red cells washed and stored in a citrate‐phosphate‐glucose‐adenine solution at pH 7.4–7.6 demonstrate excellent maintenance of adenosine triphosphate, elevation of 2,3‐diphosphoglycerate well above normal levels for more than 6 weeks, reduced hemolysis and 24‐hour in vivo survival comparable to that of cells stored in ADSOL. These results can be attributed in part to a chloride shift in which the washout of intracellular chloride is associated with an influx of OH“, which increases intracellular pH and thereby increases the rate of glycolysis. The phosphate functions primarily as a buffer to maintain both extra‐ and intracellular pH. Reducing the effective osmolality of the storage solution reduces hemolysis and improves cell morphology. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Vox Sanguinis Wiley

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Publisher
Wiley
Copyright
© 1991 S. Karger AG, Basel
ISSN
0042-9007
eISSN
1423-0410
D.O.I.
10.1111/j.1423-0410.1991.tb00880.x
Publisher site
See Article on Publisher Site

Abstract

Abstract. Red cells washed and stored in a citrate‐phosphate‐glucose‐adenine solution at pH 7.4–7.6 demonstrate excellent maintenance of adenosine triphosphate, elevation of 2,3‐diphosphoglycerate well above normal levels for more than 6 weeks, reduced hemolysis and 24‐hour in vivo survival comparable to that of cells stored in ADSOL. These results can be attributed in part to a chloride shift in which the washout of intracellular chloride is associated with an influx of OH“, which increases intracellular pH and thereby increases the rate of glycolysis. The phosphate functions primarily as a buffer to maintain both extra‐ and intracellular pH. Reducing the effective osmolality of the storage solution reduces hemolysis and improves cell morphology.

Journal

Vox SanguinisWiley

Published: Feb 1, 1991

References

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