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N. Packer, M. Lawson, D. Jardine, J. Redmond (1998)
A general approach to desalting oligosaccharides released from glycoproteinsGlycoconjugate Journal, 15
M. Pabst, J. Bondili, J. Stadlmann, L. Mach, F. Altmann (2007)
Mass + retention time = structure: a strategy for the analysis of N-glycans by carbon LC-ESI-MS and its application to fibrin N-glycans.Analytical chemistry, 79 13
J. Ahn, J. Bones, Y. Yu, P. Rudd, M. Gilar (2010)
Separation of 2-aminobenzamide labeled glycans using hydrophilic interaction chromatography columns packed with 1.7 microm sorbent.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878 3-4
H. Debray, G. Strecker, J. Montreuil (1984)
Effect of alkalis on N-glycosidic linkages of glycoproteins.Biochemical Society transactions, 12 4
Xuezheng Song, Hong Ju, Yi Lasanajak, M. Kudelka, David Smith, R. Cummings (2016)
Oxidative Release of Natural Glycans for Functional GlycomicsNature methods, 13
A. Tarentino, A. Phelan, T. Plummer (1993)
2-Iminothiolane: a reagent for the introduction of sulphydryl groups into oligosaccharides derived from asparagine-linked glycans.Glycobiology, 3 3
Jimenez-Castells (2016)
1297Glycobiology, 26
E. Hounsell, N. Pickering, M. Stoll, A. Lawson, T. Feizi (1984)
The effect of mild alkali and alkaline borohydride on the carbohydrate and peptide moieties of fetuin.Biochemical Society transactions, 12 4
Carmen Jiménez-Castells, R. Stanton, Shi Yan, P. Kosma, I. Wilson (2016)
Development of a multifunctional aminoxy-based fluorescent linker for glycan immobilization and analysis.Glycobiology, 26 12
P. Jensen, N. Karlsson, D. Kolarich, N. Packer (2012)
Structural analysis of N- and O-glycans released from glycoproteinsNature Protocols, 7
L. Likhosherstov, O. Novikova, V. Derevitskaya, N. Kochetkov (1990)
A selective method for sequential splitting of O- and N-linked glycans from N,O-glycoproteins.Carbohydrate research, 199 1
Bing Gong, Erik Hoyt, Heather Lynaugh, Irina Burnina, Renée Moore, Alissa Thompson, Huijuan Li (2013)
N-Glycosylamine-mediated isotope labeling for mass spectrometry-based quantitative analysis of N-linked glycansAnalytical and Bioanalytical Chemistry, 405
Keita Yamada, J. Hirabayashi, K. Kakehi (2013)
Analysis of O-glycans as 9-fluorenylmethyl derivatives and its application to the studies on glycan array.Analytical chemistry, 85 6
Benisek Wf, R. Ma, Cole Rd (1967)
Reductive cleavage of acylproline peptide bonds.Biochemistry, 6
D. Kolarich, Markus Windwarder, K. Alagesan, F. Altmann (2015)
Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon.Methods in molecular biology, 1321
B. Schulz, N. Packer, N. Karlsson (2002)
Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis.Analytical chemistry, 74 23
S. Ogata, K. Lloyd (1982)
Mild alkaline borohydride treatment of glycoproteins-a method for liberating both N- and O-linked carbohydrate chains.Analytical biochemistry, 119 2
D. Carlson (1968)
Structures and immunochemical properties of oligosaccharides isolated from pig submaxillary mucins.The Journal of biological chemistry, 243 3
(2017)
Determination of true ratios of different N-glycan structures in electrospray ionization mass spectrometryAnalytical and Bioanalytical Chemistry, 409
M. Lauber, Y. Yu, D. Brousmiche, Zhengmao Hua, Stephan Koza, P. Magnelli, E. Guthrie, C. Taron, K. Fountain (2015)
Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Labeling Reagent that Facilitates Sensitive Fluorescence and ESI-MS Detection.Analytical chemistry, 87 10
N. Karlsson, N. Packer (2002)
Analysis of O-linked reducing oligosaccharides released by an in-line flow system.Analytical biochemistry, 305 2
S. Argade, G Daves, H. Halbeek, Jack Alhadeff (2005)
The effect of alkaline borohydride treatment onN-linked carbohydrates of glycoproteinsGlycoconjugate Journal, 6
(2016)
Cummings
M. Pabst, Shengqian Wu, J. Grass, Alexander Kolb, C. Chiari, H. Viernstein, F. Unger, F. Altmann, S. Toegel (2010)
IL-1beta and TNF-alpha alter the glycophenotype of primary human chondrocytes in vitro.Carbohydrate research, 345 10
Guangli Yu, Yibing Zhang, Zhenqing Zhang, Letian Song, Peipei Wang, W. Chai (2010)
Effect and limitation of excess ammonium on the release of O-glycans in reducing forms from glycoproteins under mild alkaline conditions for glycomic and functional analysis.Analytical chemistry, 82 22
Haralt Leiter, J. Mucha, E. Staudacher, R. Grimm, J. Glössl, F. Altmann (1999)
Purification, cDNA Cloning, and Expression of GDP-l-Fuc:Asn-linked GlcNAc α1,3-Fucosyltransferase from Mung Beans*The Journal of Biological Chemistry, 274
Spiro (1974)
5704J. Biol. Chem., 249
R. Spiro, V. Bhoyroo (1974)
Structure of the O-glycosidically linked carbohydrate units of fetuin.The Journal of biological chemistry, 249 18
A. Tarentino, T. Plummer (1987)
Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase and endo-beta-N-acetylglucosaminidase from Flavobacterium meningosepticum.Methods in enzymology, 138
Maija-Liisa Rasilo, O. Renkonen (1981)
Mild alkaline borohydride treatment liberates N‐acetylglucosamine‐linked oligosaccharide chains of glycoproteinsFEBS Letters, 135
M. Pabst, F. Altmann (2011)
Glycan analysis by modern instrumental methodsPROTEOMICS, 11
Release of O‐glycans by reductive β‐elimination has become routine in many glyco‐analytical laboratories and concomitant release of N‐glycans has repeatedly been observed. Revisiting this somewhat forgotten mode of N‐glycan release revealed that all kinds of N‐glycans including oligomannosidic and complex‐type N‐glycans from plants with 3‐linked fucose and from mammals with or without 6‐linked fucose and with sialic acid could be recovered. However, the mass spectra of the obtained products revealed very surprising facts. Even after 16 h incubation in 1 M sodium borohydride, a large part of the glycans occurred in reducing form. Moreover, about one third emerged in the form of the stable amino‐functionalized 1‐amino‐1‐deoxy‐glycitol. When avoiding acidic conditions, considerable amounts of glycosylamine were observed. In addition, a compound with a reduced asparagine and de‐N‐acetylation products, in particular of sialylated glycans, was seen. The relative yields of the products reducing glycosylamine, reducing N‐glycan, 1‐amino‐1‐deoxy‐glycitol or glycitol could be controlled by the release conditions, foremost by temperature and borohydride concentration. Thus, chemical release of N‐glycans constitutes a cost‐saving alternative to enzymatic hydrolysis for the preparation of precursors for the production of reference compounds for various formats of N‐glycan analysis. Moreover, it allows to obtain a stable amino‐functionalized glycan derivative, which can be employed to construct glycan arrays or affinity matrices.
Proteomics – Wiley
Published: Jan 1, 2018
Keywords: ; ; ; ;
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