REDUCED VIABILITY OF VASCULAR ENDOTHELIAL CELLS BY HIGH CONCENTRATION OF ASCORBIC ACID IN VITREOUS HUMOR

REDUCED VIABILITY OF VASCULAR ENDOTHELIAL CELLS BY HIGH CONCENTRATION OF ASCORBIC ACID IN... Normal mammalian vitreous humor maintains its avascularity after regression of hyaloid vessels. Neovascularization in adults is only detected under pathological conditions which suggests that antiangiogenic factors are present in the vitreous humor. To elucidate the mechanism of vitreal angiogenic inhibition, we investigated the effect of vitreous humor on cultured vascular endothelial cells. When bovine aortic endothelial cells were cultured in the presence of bovine vitreous humor in medium, a decrease in cell viability was observed within 24h. Ascorbic acid from vitreous humor has been identified as a cell death inducing factor with high performance liquid chromatography (HPLC) and molecular mass analysis. Ascorbic acid reduced endothelial cell viability at concentrations normally present in vitreous humor. This effect was completely inhibited by antioxidants, N‐acetylcysteine and catalase. Amongst the ascorbic acid derivatives tested, ascorbic acid 2‐phosphate did not induce cell death, suggesting that the production of ascorbyl radical is required for induction of cell death. Furthermore, capillary formation in three‐dimensional collagen gel cultures characteristic of vascular endothelial cells were disrupted in the presence of ascorbic acid. Since ascorbic acid is highly concentrated in ocular tissues, especially in vitreous humor, it may function as a neovascularization inhibitor. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cell Biology International Wiley

REDUCED VIABILITY OF VASCULAR ENDOTHELIAL CELLS BY HIGH CONCENTRATION OF ASCORBIC ACID IN VITREOUS HUMOR

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Publisher
Wiley
Copyright
© The Author(s) Journal compilation © 1999 International Federation for Cell Biology
ISSN
1065-6995
eISSN
1095-8355
D.O.I.
10.1006/cbir.1999.0347
Publisher site
See Article on Publisher Site

Abstract

Normal mammalian vitreous humor maintains its avascularity after regression of hyaloid vessels. Neovascularization in adults is only detected under pathological conditions which suggests that antiangiogenic factors are present in the vitreous humor. To elucidate the mechanism of vitreal angiogenic inhibition, we investigated the effect of vitreous humor on cultured vascular endothelial cells. When bovine aortic endothelial cells were cultured in the presence of bovine vitreous humor in medium, a decrease in cell viability was observed within 24h. Ascorbic acid from vitreous humor has been identified as a cell death inducing factor with high performance liquid chromatography (HPLC) and molecular mass analysis. Ascorbic acid reduced endothelial cell viability at concentrations normally present in vitreous humor. This effect was completely inhibited by antioxidants, N‐acetylcysteine and catalase. Amongst the ascorbic acid derivatives tested, ascorbic acid 2‐phosphate did not induce cell death, suggesting that the production of ascorbyl radical is required for induction of cell death. Furthermore, capillary formation in three‐dimensional collagen gel cultures characteristic of vascular endothelial cells were disrupted in the presence of ascorbic acid. Since ascorbic acid is highly concentrated in ocular tissues, especially in vitreous humor, it may function as a neovascularization inhibitor.

Journal

Cell Biology InternationalWiley

Published: Apr 1, 1999

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