Reassessment of the Access Testosterone chemiluminescence assay and comparison with LC‐MS method

Reassessment of the Access Testosterone chemiluminescence assay and comparison with LC‐MS method INTRODUCTIONTestosterone is the principal androgen hormone, and is produced mainly in the male by the Leydig testis cells. It is also present in the female as intermediate metabolite of the estrogens synthesis and derived by androstenedione by peripheral conversion.The testosterone determination can be used in the delayed puberty, in primary or secondary hypogonadism and in some female endocrine disorders, as hirsutism, virilization and/or in polycystic ovarian syndrome.It is worth noting that, so far, no reliable routine methods for direct determination of active testosterone free form are available, and it is to date suggested the estimate of bioavailable testosterone through total testosterone determination and the mainly binding protein, the Sex Hormone Binding Globulin. However, the commonly used immunometric assays for testosterone measurement could show limited sensitivity and accuracy at clinically relevant levels.One of the reasons of these analytical drawbacks could be interferences from other steroid hormones. In particular, dehydroepiandrosterone‐sulfate (DHEAS), although structurally less similar to testosterone in respect to other steroids, could significantly cross‐react with testosterone measurements due to its relatively high circulating concentrations, on average of three orders of magnitude greater than those of the testosterone.Aim of the study is to re‐evaluate the technical characteristics of one automatic immunometric http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Laboratory Analysis Wiley

Reassessment of the Access Testosterone chemiluminescence assay and comparison with LC‐MS method

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0887-8013
eISSN
1098-2825
D.O.I.
10.1002/jcla.22286
Publisher site
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Abstract

INTRODUCTIONTestosterone is the principal androgen hormone, and is produced mainly in the male by the Leydig testis cells. It is also present in the female as intermediate metabolite of the estrogens synthesis and derived by androstenedione by peripheral conversion.The testosterone determination can be used in the delayed puberty, in primary or secondary hypogonadism and in some female endocrine disorders, as hirsutism, virilization and/or in polycystic ovarian syndrome.It is worth noting that, so far, no reliable routine methods for direct determination of active testosterone free form are available, and it is to date suggested the estimate of bioavailable testosterone through total testosterone determination and the mainly binding protein, the Sex Hormone Binding Globulin. However, the commonly used immunometric assays for testosterone measurement could show limited sensitivity and accuracy at clinically relevant levels.One of the reasons of these analytical drawbacks could be interferences from other steroid hormones. In particular, dehydroepiandrosterone‐sulfate (DHEAS), although structurally less similar to testosterone in respect to other steroids, could significantly cross‐react with testosterone measurements due to its relatively high circulating concentrations, on average of three orders of magnitude greater than those of the testosterone.Aim of the study is to re‐evaluate the technical characteristics of one automatic immunometric

Journal

Journal of Clinical Laboratory AnalysisWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ;

References

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