The squid Heterololigo bleekeri are distributed in the colder coastal waters around Japan and is an important fisheries resource ( Natsukari & Tashiro 1991 ). Heterololigo bleekeri are also often used as typical material for neuroscience. After the successful maintenance of adult H. bleekeri in a closed seawater system ( Matsumoto 1976 ), that satisfied the demand for neurophysiological studies. Later, trial culturing of this species were undertaken in which hatchlings were reared up to 114 days (Kanagawa Fisheries Experimental Station, Annual Report, 1983 (in Japanese)) and 78 days (Japan Sea Farming Association, Annual Report, 1987–1995 (in Japanese)) after hatching in an aquarium with running seawater and marine organisms that had been collected near the laboratory were provided as the main diet together with an artificial diet. Unfortunately, this was only suitable for a laboratory near the ocean. We tried to raise H. bleekeri hatchlings within a closed seawater system and succeeded rearing 2 months old juveniles. There were two material sources for the present study, that were used for the experiments completed in 2000 and in 2002 respectively. First, mature female H. bleekeri were collected by nets set in the coastal waters of southern Hokkaido on February
Aquaculture Research – Wiley
Published: Mar 1, 2005
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