Reaper‐induced apoptosis in a vertebrate system

Reaper‐induced apoptosis in a vertebrate system The reaper protein of Drosophila melanogaster has been shown to be a central regulator of apoptosis in that organism. However, it has not been shown to function in any vertebrate nor have the cellular components required for its action been defined. In this report we show that reaper can induce rapid apoptosis in vitro using an apoptotic reconstitution system derived from Xenopus eggs. Moreover, we show that a subcellular fraction enriched in mitochondria is required for this process and that reaper, acting in conjunction with cytosolic factors, can trigger mitochondrial cytochrome c release. Bcl‐2 antagonizes these effects, but high levels of reaper can overcome the Bcl‐2 block. These results demonstrate that reaper can function in a vertebrate context, suggesting that reaper‐responsive factors are conserved elements of the apoptotic program. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

Reaper‐induced apoptosis in a vertebrate system

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Abstract

The reaper protein of Drosophila melanogaster has been shown to be a central regulator of apoptosis in that organism. However, it has not been shown to function in any vertebrate nor have the cellular components required for its action been defined. In this report we show that reaper can induce rapid apoptosis in vitro using an apoptotic reconstitution system derived from Xenopus eggs. Moreover, we show that a subcellular fraction enriched in mitochondria is required for this process and that reaper, acting in conjunction with cytosolic factors, can trigger mitochondrial cytochrome c release. Bcl‐2 antagonizes these effects, but high levels of reaper can overcome the Bcl‐2 block. These results demonstrate that reaper can function in a vertebrate context, suggesting that reaper‐responsive factors are conserved elements of the apoptotic program.

Journal

The EMBO JournalWiley

Published: Mar 15, 1998

Keywords: ; ;

References

  • Cell death in health and disease: the biology and regulation of apoptosis
    Bellamy, COC; Malcomson, RDG; Harrison, DJ; Wyllie, AH
  • The cell‐death machine
    Chinnaiyan, AM; Dixit, VM
  • Crk is required for apoptosis in Xenopus egg extracts
    Evans, EK; Lu, W; Strum, SL; Mayer, BJ; Kornbluth, S
  • Cytochrome c activation of CPP32‐like proteolysis plays a critical role in a Xenopus cell‐free apoptosis system
    Kluck, RM; Martin, SJ; Hoffman, BM; Zhou, JS; Green, DR; Newmeyer, DD
  • Bcl‐2 and the regulation of programmed cell death
    Reed, JC
  • Cell killing by the Drosophila gene reaper
    White, K; Tahaoglu, E; Steller, H
  • Molecular control of life and death
    Yuan, J

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