RBMX is a component of the centromere noncoding RNP complex involved in cohesion regulation

RBMX is a component of the centromere noncoding RNP complex involved in cohesion regulation Satellite I RNA, a noncoding (nc)RNA transcribed from repetitive regions in human centromeres, binds to Aurora kinase B and forms a ncRNP complex required for chromosome segregation. To examine its function in this process, we purified satellite I ncRNP complex from nuclear extracts prepared from asynchronized or mitotic (M) phase‐arrested HeLa cells and then carried out LC/MS to identify proteins bound to satellite I RNA. RBMX (RNA‐binding motif protein, X‐linked), which was isolated from M phase‐arrested cells, was selected for further characterization. We found that RBMX associates with satellite I RNA only during M phase. Knockdown of RBMX induced premature separation of sister chromatid cohesion and abnormal nuclear division. Likewise, knockdown of satellite I RNA also caused premature separation of sister chromatids during M phase. The amounts of RBMX and Sororin, a cohesion regulator, were reduced in satellite I RNA‐depleted cells. These results suggest that satellite I RNA plays a role in stabilizing RBMX and Sororin in the ncRNP complex to maintain proper sister chromatid cohesion. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes to Cells Wiley

RBMX is a component of the centromere noncoding RNP complex involved in cohesion regulation

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd
ISSN
1356-9597
eISSN
1365-2443
D.O.I.
10.1111/gtc.12562
Publisher site
See Article on Publisher Site

Abstract

Satellite I RNA, a noncoding (nc)RNA transcribed from repetitive regions in human centromeres, binds to Aurora kinase B and forms a ncRNP complex required for chromosome segregation. To examine its function in this process, we purified satellite I ncRNP complex from nuclear extracts prepared from asynchronized or mitotic (M) phase‐arrested HeLa cells and then carried out LC/MS to identify proteins bound to satellite I RNA. RBMX (RNA‐binding motif protein, X‐linked), which was isolated from M phase‐arrested cells, was selected for further characterization. We found that RBMX associates with satellite I RNA only during M phase. Knockdown of RBMX induced premature separation of sister chromatid cohesion and abnormal nuclear division. Likewise, knockdown of satellite I RNA also caused premature separation of sister chromatids during M phase. The amounts of RBMX and Sororin, a cohesion regulator, were reduced in satellite I RNA‐depleted cells. These results suggest that satellite I RNA plays a role in stabilizing RBMX and Sororin in the ncRNP complex to maintain proper sister chromatid cohesion.

Journal

Genes to CellsWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ;

References

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