Rapid stimulation of a soybean protein‐serine kinase that phosphorylates a novel bZIP DNA‐binding protein, G/HBF‐1, during the induction of early transcription‐dependent defenses

Rapid stimulation of a soybean protein‐serine kinase that phosphorylates a novel bZIP... The G‐box (CACGTG) and H‐box (CCTACC) cis elements function in the activation of phenylpropanoid biosynthetic genes involved in the elaboration of lignin precursors, phytoalexins and the secondary signal salicylic acid as early responses to pathogen attack. We have isolated a soybean cDNA encoding a novel bZIP protein, G/HBF‐1, which binds to both the G‐box and adjacent H‐box in the proximal region of the chalcone synthase chs15 promoter. While G/HBF‐1 transcript and protein levels do not increase during the induction of phenylpropanoid biosynthetic genes, G/HBF‐1 is phosphorylated rapidly in elicited soybean cells, almost exclusively on serine residues. Using recombinant G/HBF‐1 as a substrate, we identified a cytosolic protein‐serine kinase that is rapidly and transiently stimulated in cells elicited with either glutathione or an avirulent strain of the soybean pathogen Pseudomonas syringae pv. glycinea. Phosphorylation of G/HBF‐1 in vitro enhances binding to the chs15 promoter and we conclude that stimulation of G/HBF‐1 kinase activity and G/HBF‐1 phosphorylation are terminal events in a signal pathway for activation of early transcription‐dependent plant defense responses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

Rapid stimulation of a soybean protein‐serine kinase that phosphorylates a novel bZIP DNA‐binding protein, G/HBF‐1, during the induction of early transcription‐dependent defenses

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Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc
ISSN
0261-4189
eISSN
1460-2075
D.O.I.
10.1093/emboj/16.4.726
Publisher site
See Article on Publisher Site

Abstract

The G‐box (CACGTG) and H‐box (CCTACC) cis elements function in the activation of phenylpropanoid biosynthetic genes involved in the elaboration of lignin precursors, phytoalexins and the secondary signal salicylic acid as early responses to pathogen attack. We have isolated a soybean cDNA encoding a novel bZIP protein, G/HBF‐1, which binds to both the G‐box and adjacent H‐box in the proximal region of the chalcone synthase chs15 promoter. While G/HBF‐1 transcript and protein levels do not increase during the induction of phenylpropanoid biosynthetic genes, G/HBF‐1 is phosphorylated rapidly in elicited soybean cells, almost exclusively on serine residues. Using recombinant G/HBF‐1 as a substrate, we identified a cytosolic protein‐serine kinase that is rapidly and transiently stimulated in cells elicited with either glutathione or an avirulent strain of the soybean pathogen Pseudomonas syringae pv. glycinea. Phosphorylation of G/HBF‐1 in vitro enhances binding to the chs15 promoter and we conclude that stimulation of G/HBF‐1 kinase activity and G/HBF‐1 phosphorylation are terminal events in a signal pathway for activation of early transcription‐dependent plant defense responses.

Journal

The EMBO JournalWiley

Published: Feb 15, 1997

References

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