Quantitative direct probe method for the detection of parvovirus B19

Quantitative direct probe method for the detection of parvovirus B19 Parvovirus B19 infection is associated with anemia and spontaneous abortions. While many qualitative assays are available, a few molecular‐based quantitative methods have been described. This study reports the development and optimization of a quantitative direct‐probe method for the detection of Parvovirus B19 DNA. Different concentrations of RNA probes were used to identify the optimal conditions for hybridizing to the target DNA. Detection of DNA was linear between concentrations of 2 ng/ml to 200 pg/ml. Because this method requires no enzymatic amplification, it is not susceptible to amplifier contamination or enzymatic inhibitors, and it can be applied to serum samples or paraffin‐embedded tissue. J. Clin. Lab. Anal. 14:38–41, 2000. © 2000 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Laboratory Analysis Wiley

Quantitative direct probe method for the detection of parvovirus B19

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Publisher
Wiley
Copyright
Copyright © 2000 Wiley‐Liss, Inc.
ISSN
0887-8013
eISSN
1098-2825
D.O.I.
10.1002/(SICI)1098-2825(2000)14:1<38::AID-JCLA8>3.0.CO;2-N
Publisher site
See Article on Publisher Site

Abstract

Parvovirus B19 infection is associated with anemia and spontaneous abortions. While many qualitative assays are available, a few molecular‐based quantitative methods have been described. This study reports the development and optimization of a quantitative direct‐probe method for the detection of Parvovirus B19 DNA. Different concentrations of RNA probes were used to identify the optimal conditions for hybridizing to the target DNA. Detection of DNA was linear between concentrations of 2 ng/ml to 200 pg/ml. Because this method requires no enzymatic amplification, it is not susceptible to amplifier contamination or enzymatic inhibitors, and it can be applied to serum samples or paraffin‐embedded tissue. J. Clin. Lab. Anal. 14:38–41, 2000. © 2000 Wiley‐Liss, Inc.

Journal

Journal of Clinical Laboratory AnalysisWiley

Published: Jan 1, 2000

References

  • Automated detection of digoxigenin‐labeled B19 parvovirus amplicons by capture hybridization assay
    Zerbini, Zerbini; Gibellini, Gibellini; Musiani, Musiani; Venturoli, Venturoli; Gallinella, Gallinella; Gentilomi, Gentilomi
  • Synthetic oligonucleotide cocktail, as probes for detection of human parvovirus B19
    Cubie, Cubie; Grzybowski, Grzybowski; da Silva, da Silva; Duncan, Duncan; Brown, Brown; Smith, Smith

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