Quantitation of hepatitis C virus RNA in serum of asymptomatic blood donors and patients with type C chronic liver disease

Quantitation of hepatitis C virus RNA in serum of asymptomatic blood donors and patients with... We describe a method for quantifying hepatitis C virus RNA in serum. This competitive assay combines reverse transcription and polymerase chain reaction and is based on coamplification of the target RNA with known amounts of synthetic mutated RNA. We tested serum samples from 104 hepatitis C virus carriers (9 asymptomatic blood donors and 95 patients with type C chronic liver disease) to determine the relationship between the replicative level of hepatitis C virus and various stages of the carrier states. The amount of circulating hepatitis C virus RNA ranged from 104 to 109.5 genomes/ml serum. The titer of hepatitis C virus RNA (logarithmic transformed copy numbers of RNA per milliliter of serum) was lower in asymptomatic blood donors (5.4 ± 2.0) and in patients with chronic persistent hepatitis (7.3 ± 1.1) than in patients with chronic active hepatitis (7.9 ± 0.8), cirrhosis (7.8 ± 0.7) or hepatocellular carcinoma (7.9 ± 0.7). The titer of hepatitis C virus RNA was significantly lower in carriers younger than 40 yr old and correlated positively with the logarithmic transformed serum ALT level. Logistic regression showed that age and titer of hepatitis C virus RNA correlated independently with the stages of liver disease. These results showed that the replicative level of hepatitis C virus is higher in advanced liver disease and that elevation of viral replication may play an important role in liver injury and progression of liver disease. This competitive assay is useful in evaluating the state of viral replication in hepatitis C virus infection. (HEPATOLOGY 1993;17:545–550.) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Hepatology Wiley

Quantitation of hepatitis C virus RNA in serum of asymptomatic blood donors and patients with type C chronic liver disease

Loading next page...
 
/lp/wiley/quantitation-of-hepatitis-c-virus-rna-in-serum-of-asymptomatic-blood-hDxeH9prrT
Publisher
Wiley
Copyright
Copyright © 1993 American Association for the Study of Liver Diseases
ISSN
0270-9139
eISSN
1527-3350
D.O.I.
10.1002/hep.1840170404
Publisher site
See Article on Publisher Site

Abstract

We describe a method for quantifying hepatitis C virus RNA in serum. This competitive assay combines reverse transcription and polymerase chain reaction and is based on coamplification of the target RNA with known amounts of synthetic mutated RNA. We tested serum samples from 104 hepatitis C virus carriers (9 asymptomatic blood donors and 95 patients with type C chronic liver disease) to determine the relationship between the replicative level of hepatitis C virus and various stages of the carrier states. The amount of circulating hepatitis C virus RNA ranged from 104 to 109.5 genomes/ml serum. The titer of hepatitis C virus RNA (logarithmic transformed copy numbers of RNA per milliliter of serum) was lower in asymptomatic blood donors (5.4 ± 2.0) and in patients with chronic persistent hepatitis (7.3 ± 1.1) than in patients with chronic active hepatitis (7.9 ± 0.8), cirrhosis (7.8 ± 0.7) or hepatocellular carcinoma (7.9 ± 0.7). The titer of hepatitis C virus RNA was significantly lower in carriers younger than 40 yr old and correlated positively with the logarithmic transformed serum ALT level. Logistic regression showed that age and titer of hepatitis C virus RNA correlated independently with the stages of liver disease. These results showed that the replicative level of hepatitis C virus is higher in advanced liver disease and that elevation of viral replication may play an important role in liver injury and progression of liver disease. This competitive assay is useful in evaluating the state of viral replication in hepatitis C virus infection. (HEPATOLOGY 1993;17:545–550.)

Journal

HepatologyWiley

Published: Apr 1, 1993

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off