Quantification of dsRNA using stable isotope labeling dilution liquid chromatography/mass spectrometry

Quantification of dsRNA using stable isotope labeling dilution liquid chromatography/mass... INTRODUCTIONExploitation of the RNA interference (RNAi) pathway to block the expression of specific genes holds considerable promise for the development of novel RNAi‐based insect management strategies. There are a wide range of future potential applications of RNAi to control agricultural insect pests as well as its use for prevention of diseases in beneficial insects. Recent developments in RNAi have created a need for cost‐effective, large‐scale synthesis of double‐stranded RNA (dsRNA), which in turn requires robust analytical techniques to fully characterise and accurately quantify dsRNA prior to RNAi applications. A wide range of dsRNA products can be generated either via bacterial expression systems, in planta or in vitro transcription. The development of suitable analytical methods to characterise the dsRNA products remains a significant challenge.E. coli‐mediated delivery of dsRNA has been reported in C. elegans, planarians, Entamoeba histolytica and Spodoptera exigua. Furthermore, a number of RNAi‐based insect management strategies have also employed the ingestion of bacteria‐expressing dsRNA, application of chemically synthesised dsRNA and transgenic plants expressing dsRNA. To ensure RNAi gene silencing using the above approaches, it is important to both produce and deliver the required amounts of dsRNA. Therefore, the necessary analytical tools to quantify the dsRNA are important to http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Rapid Communications in Mass Spectrometry Wiley

Quantification of dsRNA using stable isotope labeling dilution liquid chromatography/mass spectrometry

Loading next page...
 
/lp/wiley/quantification-of-dsrna-using-stable-isotope-labeling-dilution-liquid-UXxjGQe0rZ
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons, Ltd.
ISSN
0951-4198
eISSN
1097-0231
D.O.I.
10.1002/rcm.8074
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONExploitation of the RNA interference (RNAi) pathway to block the expression of specific genes holds considerable promise for the development of novel RNAi‐based insect management strategies. There are a wide range of future potential applications of RNAi to control agricultural insect pests as well as its use for prevention of diseases in beneficial insects. Recent developments in RNAi have created a need for cost‐effective, large‐scale synthesis of double‐stranded RNA (dsRNA), which in turn requires robust analytical techniques to fully characterise and accurately quantify dsRNA prior to RNAi applications. A wide range of dsRNA products can be generated either via bacterial expression systems, in planta or in vitro transcription. The development of suitable analytical methods to characterise the dsRNA products remains a significant challenge.E. coli‐mediated delivery of dsRNA has been reported in C. elegans, planarians, Entamoeba histolytica and Spodoptera exigua. Furthermore, a number of RNAi‐based insect management strategies have also employed the ingestion of bacteria‐expressing dsRNA, application of chemically synthesised dsRNA and transgenic plants expressing dsRNA. To ensure RNAi gene silencing using the above approaches, it is important to both produce and deliver the required amounts of dsRNA. Therefore, the necessary analytical tools to quantify the dsRNA are important to

Journal

Rapid Communications in Mass SpectrometryWiley

Published: Jan 15, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off