Pyruvate and lactate protect striatal neurons against N‐methyl‐ d ‐aspartate‐induced neurotoxicity

Pyruvate and lactate protect striatal neurons against N‐methyl‐ d ‐aspartate‐induced... A sustained release of glutamate contributes to neuronal loss during cerebral ischaemia. Using cultured mouse striatal neurons, we observed that glucose deprivation, which occurs in this pathological process, enhanced the N‐Methyl‐d‐aspartate (NMDA)‐ or α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionate (AMPA)‐induced neurotoxicity. The end products of glycolysis, lactate and pyruvate, strongly protected neurons from these neurotoxic effects. The neuroprotective effect of pyruvate (which is more prominent in the absence of glucose) was not related to its ability to react with H2O2 by a decarboxylation process. Pyruvate and l‐lactate strongly counteracted the deep decrease in the neuronal ATP content induced by NMDA, indicating that they might protect striatal neurons by rescuing cellular energy charge. Addition of MK‐801 after the NMDA withdrawal completely protected neurons, suggesting that NMDA neurotoxicity resulted from a delayed NMDA receptor activation probably linked to a delayed release of an endogenous agonist in the extracellular medium. The strong accumulation of extracellular glutamate which was found in both sham and NMDA‐treated cultures was markedly decreased by pyruvate. Thus, pyruvate might also exert its protecting activity by decreasing the delayed accumulation of glutamate which seemed to be neurotoxic only after a preexposure of neurons to NMDA. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

Pyruvate and lactate protect striatal neurons against N‐methyl‐ d ‐aspartate‐induced neurotoxicity

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Publisher
Wiley
Copyright
Copyright © 1999 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
D.O.I.
10.1046/j.1460-9568.1999.00745.x
Publisher site
See Article on Publisher Site

Abstract

A sustained release of glutamate contributes to neuronal loss during cerebral ischaemia. Using cultured mouse striatal neurons, we observed that glucose deprivation, which occurs in this pathological process, enhanced the N‐Methyl‐d‐aspartate (NMDA)‐ or α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionate (AMPA)‐induced neurotoxicity. The end products of glycolysis, lactate and pyruvate, strongly protected neurons from these neurotoxic effects. The neuroprotective effect of pyruvate (which is more prominent in the absence of glucose) was not related to its ability to react with H2O2 by a decarboxylation process. Pyruvate and l‐lactate strongly counteracted the deep decrease in the neuronal ATP content induced by NMDA, indicating that they might protect striatal neurons by rescuing cellular energy charge. Addition of MK‐801 after the NMDA withdrawal completely protected neurons, suggesting that NMDA neurotoxicity resulted from a delayed NMDA receptor activation probably linked to a delayed release of an endogenous agonist in the extracellular medium. The strong accumulation of extracellular glutamate which was found in both sham and NMDA‐treated cultures was markedly decreased by pyruvate. Thus, pyruvate might also exert its protecting activity by decreasing the delayed accumulation of glutamate which seemed to be neurotoxic only after a preexposure of neurons to NMDA.

Journal

European Journal of NeuroscienceWiley

Published: Sep 1, 1999

References

  • Elevation of the extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischemia by intracerebral microdialysis.
    Benveniste, Benveniste; Drejer, Drejer; Schousboe, Schousboe; Diemer, Diemer
  • The excitatory amino acid receptors: their classes, pharmacology and distinct properties in the function of the central nervous system.
    Monaghan, Monaghan; Bridges, Bridges; Cotman, Cotman
  • Is protein kinase C activity required for the N ‐methyl‐d‐aspartate evoked rise in cytosolic Ca 2+ in mouse striatal neurons?
    Murphy, Murphy; Cordier, Cordier; Glowinski, Glowinski; Prémont, Prémont
  • Glutamate and the pathophysiology of hypoxic‐ischemic brain damage.
    Rothman, Rothman; Olney, Olney
  • Cytotoxic effect of brain macrophages on developing neurons.
    Théry, Théry; Chamak, Chamak; Mallat, Mallat
  • Na + –Ca 2+ exchange currents in cortical neurons: concomitant forward and reverse operation and effect of glutamate.
    Yu, Yu; Choi, Choi

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