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References (12)
-
O. Lowry, N. Rosebrough, A. Farr, R. Randall (1951)
Protein measurement with the Folin phenol reagent.The Journal of biological chemistry, 193 1
-
F. Westall, V. Lennon, D. Gospodarowicz (1978)
Brain-derived fibroblast growth factor: identity with a fragment of the basic protein of myelin.Proceedings of the National Academy of Sciences of the United States of America, 75 10
-
D. Gospodarowicz (1974)
Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growthNature, 249
-
D. Gospodarowicz (1975)
Purification of a fibroblast growth factor from bovine pituitary.The Journal of biological chemistry, 250 7
-
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
-
R. Bradshaw, J. Rubin (1980)
Polypeptide growth factors: some structural and mechanistic considerations.Journal of supramolecular structure, 14 2
-
(1975)
In Vitro 14235 , 1978 -
Jeffrey Rubin, I. Mariz, John Jacobs, William Daughaday, Ralph Bradshaw (1982)
Isolation and partial sequence analysis of rat basic somatomedin.Endocrinology, 110 3
-
D. Rodbard, A. Chrambach (1971)
Estimation of molecular radius, free mobility, and valence using polyacylamide gel electrophoresis.Analytical biochemistry, 40 1
-
K. Thomas, M. Riley, S. Lemmon, N. Baglan, R. Bradshaw (1980)
Brain fibroblast growth factor: nonidentity with myelin basic protein fragments.The Journal of biological chemistry, 255 12
-
T. Maciag, J. Cerundolo, S. Ilsley, P. Kelley, R. Forand (1979)
An endothelial cell growth factor from bovine hypothalamus: identification and partial characterization.Proceedings of the National Academy of Sciences of the United States of America, 76 11
-
D. Gospodarowicz, H. Bialecki, G. Greenburg (1978)
Purification of the fibroblast growth factor activity from bovine brain.The Journal of biological chemistry, 253 10
- Publisher
- Wiley
- Copyright
- Copyright © 1983 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0730-2312
- eISSN
- 1097-4644
- DOI
- 10.1002/jcb.240210302
- pmid
- 6630303
- Publisher site
- See Article on Publisher Site
Abstract
A purification procedure and partial characterization of bovine pituitary fibroblast growth factor (FGF) are described. The steps of the published methods [3,4] which yield inhomogeneous material, were retained, with modifications. The final isolation, with an additional purification of ∼20‐fold, was achieved by electro‐phoresis in polyacrylamide gels at acid pH. The mitogenic peptide has a molecular weight of 14,500–15,00 as determined on SDS gels, chromatographs as a monomer in nondenaturing conditions, and is active at the picomolar level in effecting the incorporation of 3H‐thymidine in Balb/c 3T3 cells. A preliminary amino acid composition is presented.
Journal
Journal of Cellular Biochemistry – Wiley
Published: Jan 1, 1983
Keywords: ; ;