Protein transfer into the recipient cell during bacterial conjugation: studies with F and RP4

Protein transfer into the recipient cell during bacterial conjugation: studies with F and RP4 Summary Transfer of donor cell proteins to the recipient bacterium was examined in F‐ and RP4‐mediated conjugation. Transfer of a 120kD polypeptide, identified as the larger product of the plasmid DNA primase gene, was readily detected during RP4‐promoted conjugation. The protein was transmitted to the cytoplasm of the recipient, presumably complexed to the transferred ssDNA. F DNA was transferred without detectable association with any cytoplasmic tra protein or with the ssDNA‐binding protein encoded by the plasmid. However, a 92kD protein, possibly F TraD product, was transmitted to the membrane fraction of the recipient cell. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Microbiology Wiley

Protein transfer into the recipient cell during bacterial conjugation: studies with F and RP4

Molecular Microbiology, Volume 4 (7) – Jul 1, 1990

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Publisher
Wiley
Copyright
Copyright © 1990 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0950-382X
eISSN
1365-2958
DOI
10.1111/j.1365-2958.1990.tb00695.x
Publisher site
See Article on Publisher Site

Abstract

Summary Transfer of donor cell proteins to the recipient bacterium was examined in F‐ and RP4‐mediated conjugation. Transfer of a 120kD polypeptide, identified as the larger product of the plasmid DNA primase gene, was readily detected during RP4‐promoted conjugation. The protein was transmitted to the cytoplasm of the recipient, presumably complexed to the transferred ssDNA. F DNA was transferred without detectable association with any cytoplasmic tra protein or with the ssDNA‐binding protein encoded by the plasmid. However, a 92kD protein, possibly F TraD product, was transmitted to the membrane fraction of the recipient cell.

Journal

Molecular MicrobiologyWiley

Published: Jul 1, 1990

References

  • The ssb gene of plasmid Collb‐P9
    Howland, Howland; Rees, Rees; Barth, Barth; Wilkins, Wilkins
  • The conjugation system of F, the fertility factor of Escherichia coli
    Ippen‐Ihler, Ippen‐Ihler; Minkley, Minkley

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