Proliferation of glial‐derived cells in defined media

Proliferation of glial‐derived cells in defined media A serum‐free defined medium has been formulated that supports proliferation and morphologic differentiation of U‐251 MGsp human and C6‐2BD rat glioma cells. This defined medium consists of a basal medium supplemented with transferrin, fibroblast growth factor, hydrocortisone, selenium, biotin, and fibronectin (G2 medium). When U‐251 cells were plated in G2 medium on poly‐D‐lysine precoated dishes, their growth rate was 77% and final cell density was 82% of serum‐grown counterparts. The growth rate of C6 cells in G2 medium was 67% compared to cells cultured in serum supplemented medium. Although G2 medium supported the growth of human and rat glioma cells, LA‐N‐1 human neuroblastoma and WI‐38 human fibroblast cells showed no increase in cell number when grown in G2 medium compared to basal medium. A similar formulation (G3 medium), lacking fibroblast growth factor and hydrocortisone, supported the proliferation of RN‐22 rat schwannoma cells. Morphologic differences were observed between cells grown in the presence of serum and in defined media. All three glial cell lines changed from a flattened shape in serum supplemented medium to a more spherical appearance in defined medium. In addition, both U‐251 and C6 cells developed numerous processes, some reaching several cell diameters in length. These defined media will facilitate studies of the growth and differentiation of glial‐derived cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

Proliferation of glial‐derived cells in defined media

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Publisher
Wiley
Copyright
Copyright © 1982 Alan R. Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
D.O.I.
10.1002/jnr.490070212
Publisher site
See Article on Publisher Site

Abstract

A serum‐free defined medium has been formulated that supports proliferation and morphologic differentiation of U‐251 MGsp human and C6‐2BD rat glioma cells. This defined medium consists of a basal medium supplemented with transferrin, fibroblast growth factor, hydrocortisone, selenium, biotin, and fibronectin (G2 medium). When U‐251 cells were plated in G2 medium on poly‐D‐lysine precoated dishes, their growth rate was 77% and final cell density was 82% of serum‐grown counterparts. The growth rate of C6 cells in G2 medium was 67% compared to cells cultured in serum supplemented medium. Although G2 medium supported the growth of human and rat glioma cells, LA‐N‐1 human neuroblastoma and WI‐38 human fibroblast cells showed no increase in cell number when grown in G2 medium compared to basal medium. A similar formulation (G3 medium), lacking fibroblast growth factor and hydrocortisone, supported the proliferation of RN‐22 rat schwannoma cells. Morphologic differences were observed between cells grown in the presence of serum and in defined media. All three glial cell lines changed from a flattened shape in serum supplemented medium to a more spherical appearance in defined medium. In addition, both U‐251 and C6 cells developed numerous processes, some reaching several cell diameters in length. These defined media will facilitate studies of the growth and differentiation of glial‐derived cells.

Journal

Journal of Neuroscience ResearchWiley

Published: Jan 1, 1982

References

  • Effect of fibroblast growth factor on the division and fusion of bovine myoblasts
    Gospodarowicz, Gospodarowicz; Weseman, Weseman; Moran, Moran; Lindstrom, Lindstrom
  • Factors in the rat submaxillary gland that stimulate growth of cultured glioma cells: Identification and partial characterization
    McClure, McClure; Ohasa, Ohasa; Sato, Sato
  • The effect of biotin and fatty acids on SV3T3 cell growth in the presence of normal calf serum
    Messmer, Messmer; Young, Young
  • Selenium biochemistry
    Stadtman, Stadtman
  • Continuous culture of rat C6 glioma in serum‐free medium
    Wolfe, Wolfe; Sato, Sato; McClure, McClure

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