Prevascularization of natural nanofibrous extracellular matrix for engineering completely biological three‐dimensional prevascularized tissues for diverse applications

Prevascularization of natural nanofibrous extracellular matrix for engineering completely... Self‐sustainability after implantation is one of the critical obstacles facing large engineered tissues. A preformed functional vascular network provides an effective solution for solving the mass transportation problem. With the support of mural cells, endothelial cells (ECs) can form microvessels within engineered tissues. As an important mural cell, human mesenchymal stem cells (hMSCs) not only stabilize the engineered microvessel network, but also preserve their multi‐potency when grown under optimal culture conditions. A prevascularized hMSC/extracellular matrix (ECM) sheet fabricated by the combination of hMSCs, ECs and a naturally derived nanofibrous ECM scaffold offers great opportunity for engineering mechanically strong and completely biological three‐dimensional prevascularized tissues. The objective of this study was to create a prevascularized hMSC/ECM sheet by co‐culturing ECs and hMSCs on a nanofibrous ECM scaffold. Physiologically low oxygen (2% O2) was introduced during the 7 day hMSC culture to preserve the stemness of hMSCs and thereby their capability to secrete angiogenic factors. The ECs were then included to form microvessels under normal oxygen (20% O2) for up to 7 days. The results showed that a branched and mature vascular network was formed in the co‐culture condition. Angiogenic factors vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and angiopoietin‐1 (Ang‐1) were significantly increased by low‐oxygen culture of hMSCs, which further stabilized and supported the maturation of microvessels. A differentiation assay of the prevascularized ECM scaffold demonstrated a retained hMSC multi‐potency in the hypoxia cultured samples. The prevascularized hMSC/ECM sheet holds great promise for engineering three‐dimensional prevascularized tissues for diverse applications. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Tissue Engineering and Regenerative Medicine Wiley

Prevascularization of natural nanofibrous extracellular matrix for engineering completely biological three‐dimensional prevascularized tissues for diverse applications

Loading next page...
 
/lp/wiley/prevascularization-of-natural-nanofibrous-extracellular-matrix-for-XxYJLumwop
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons, Ltd.
ISSN
1932-6254
eISSN
1932-7005
D.O.I.
10.1002/term.2512
Publisher site
See Article on Publisher Site

Abstract

Self‐sustainability after implantation is one of the critical obstacles facing large engineered tissues. A preformed functional vascular network provides an effective solution for solving the mass transportation problem. With the support of mural cells, endothelial cells (ECs) can form microvessels within engineered tissues. As an important mural cell, human mesenchymal stem cells (hMSCs) not only stabilize the engineered microvessel network, but also preserve their multi‐potency when grown under optimal culture conditions. A prevascularized hMSC/extracellular matrix (ECM) sheet fabricated by the combination of hMSCs, ECs and a naturally derived nanofibrous ECM scaffold offers great opportunity for engineering mechanically strong and completely biological three‐dimensional prevascularized tissues. The objective of this study was to create a prevascularized hMSC/ECM sheet by co‐culturing ECs and hMSCs on a nanofibrous ECM scaffold. Physiologically low oxygen (2% O2) was introduced during the 7 day hMSC culture to preserve the stemness of hMSCs and thereby their capability to secrete angiogenic factors. The ECs were then included to form microvessels under normal oxygen (20% O2) for up to 7 days. The results showed that a branched and mature vascular network was formed in the co‐culture condition. Angiogenic factors vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and angiopoietin‐1 (Ang‐1) were significantly increased by low‐oxygen culture of hMSCs, which further stabilized and supported the maturation of microvessels. A differentiation assay of the prevascularized ECM scaffold demonstrated a retained hMSC multi‐potency in the hypoxia cultured samples. The prevascularized hMSC/ECM sheet holds great promise for engineering three‐dimensional prevascularized tissues for diverse applications.

Journal

Journal of Tissue Engineering and Regenerative MedicineWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial