Sodium lauryl sulphate (SLS) is a common detergent known to cause inflammatory reactions in skin. SLS‐induced toxicity is one of the most widely representative models for evaluating surfactant‐induced skin irritation. Cutaneous allergic inflammation can be modulated by neurotransmitters of sensory nerve fibres, such as substance P (SP) and calcitonin gene‐related peptides. Skin SP is produced by keratinocytes and released by afferent C‐type nerve fibres. Keratinocytes also express the neurokinin receptor, NK‐1, which is an SP receptor. SP can modulate skin inflammation. For example, SP and insulin‐like growth factor was shown to promote barrier function in corneal epithelial and human epidermoid carcinoma cells through synergistic induction, resulting in increased expression of tight‐junction protein zonula occludens‐1. In denervated corneas, weakened epithelial attachment was strengthened by E‐cadherin expression induced by SP receptor stimulation.ReportWe investigated the viability and cell–cell adhesion in SLS‐treated human keratinocytes with pretreatment, cotreatment and post‐treatment with SP. Normal human keratinocytes (NHKs) were isolated from the foreskins of healthy donors (institutional review board no. KPH 2009–01) aged 1, 9 and 11 years old. The pretreatment NHKs were cultured initially with or without SP for 24 h, then the medium was changed to SLS‐containing medium and the cells cultured for a
Clinical & Experimental Dermatology – Wiley
Published: Jan 1, 2018
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