“Whoa! It’s like Spotify but for academic articles.”

Instant Access to Thousands of Journals for just $40/month

Try 2 weeks free now

Precursor‐Directed Biosynthesis of 6‐Deoxyerythronolide B Analogs in Streptomyces coelicolor: Understanding Precursor Effects

A fermentation process employing precursor‐directed biosynthesis is being developed for the manufacture of 6‐deoxyerythronolide B (6‐dEB) analogues. Through a plasmid‐based system in Streptomyces coelicolor, 6‐dEB synthesis is catalyzed by 6‐dEB synthase (DEBS). 6‐dEB synthesis is abolished by inactivation of the ketosynthase (KS) 1 domain of DEBS but can be restored by providing synthetic activated diketides. Because of its inherent catalytic flexibility, the KS1‐deficient DEBS is capable of utilizing unnatural diketides to form various 13‐substituted 6‐dEBs. Here we characterize process variables associated with diketide feeding in shake‐flask experiments. 13‐R‐6‐dEB production was found to depend strongly on diketide feed concentrations, on the growth phase of cultures at feeding time, and on the R‐group present in the diketide moiety. In all cases a major portion of the fed diketides was degraded by the cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Progress Wiley

Loading next page...

You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy unlimited access and
personalized recommendations from
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $40/month

Try 2 weeks free now

Explore the DeepDyve Library

How DeepDyve Works

Spend time researching, not time worrying you’re buying articles that might not be useful.

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from Springer, Elsevier, Nature, IEEE, Wiley-Blackwell and more.

All the latest content is available, no embargo periods.

See the journals in your area

Simple and Affordable Pricing

14-day free trial. Cancel anytime, with a 30-day money-back guarantee.

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Best Deal — 25% off

Annual Plan

  • All the features of the Professional Plan, but for 25% off!
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

billed annually