Loading next page...
/lp/wiley/post-polymerization-modification-of-a-new-reactive-monolith-for-FLhUwpUmPk
- Publisher site
- See Article on Publisher Site
Abstract
A reactive monolith based on the polymerization of 3‐chloro‐2‐hydroxypropyl methacrylate, (HPMA‐Cl), with a crosslinking agent, ethylene glycol dimethacrylate (EDMA), was synthesized and post‐functionalized with a macromolecular ligand polyethyleneimine. Monolithic columns with controlled permeability and pore structure were prepared by free radical polymerization in the presence of a binary porogenic mixture of isopropanol and decanol. The presence of chloropropyl functionality in the pristine monolith allowed the synthesis of a post‐fuctionalized monolith carrying cationic groups that was used to control the magnitude of electroosmotic flow (EOF) in electrochromatographic separation. In the synthesis of pristine monoliths, the feed concentration of functional monomer (ie, HPMA‐Cl) was changed between 30 and 60 v/v % for obtaining cationic monoliths providing satisfactory electrochromatographic separation. The best electrochromatographic performance was obtained with the polyethyleneimine functionalized monolith prepared by using the pristine monolith obtained by 60% (v/v) monomer concentration. This monolith was used in reversed phase and hydrophilic interaction capillary electrochromatography modes for the separation of alkylbenzenes, polycyclic aromatic hydrocarbons, phenols, and nucleosides, using mobile phases with low acetonitrile (ACN) contents ranging between 20% and 35% (v/v). This ACN range was remarkably lower than the content of ACN used on the hydrophilic polymethacrylate‐based monoliths reported previously (ie, >90%). The plate heights up to 5.3 μm were obtained for the separation of nucleosides with the environmental friendly mobile phases whose ACN contents were also remarkably lower than that of similar polymethacrylate‐based monoliths.
Journal
Polymers for Advanced Technologies – Wiley
Published: Jan 1, 2018
Keywords: ; ; ; ;
Recommended Articles
Loading...
References
-
Hydrophilic‐interaction chromatography for the separation of peptides, nucleic acids and other polar compoundsAlpert, AJ
-
Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation techniqueBuszewski, B; Noga, S
-
Separation efficiencies in hydrophilic interaction chromatographyIkegami, T; Tomomatsu, K; Takubo, H; Horie, K; Tanaka, N
-
Hydroxymethyl methacrylate‐based monolithic columns designed for separation of oligonucleotides in hydrophilic‐interaction capillary liquid chromatographyHoldsvendova, P; Suchankova, J; Buncek, M; Backovska, V; Coufal, P
-
Neutral polar methacrylate‐based monoliths for normal phase nano‐LC and CEC of polar species including N‐glycansZhong, H; El Rassi, Z
-
Preparation and evaluation of rigid porous polyacrylamide‐based strong cation‐exchange monolithic columns for capillary electrochromatographyDong, J; Ou, J; Dong, X; Wu, R; Ye, M; Zou, H
-
Polar stationary phases for capillary electrochromatographyHoegger, D; Freitag, R
-
Methacrylate‐based monolithic column with mixed‐mode hydrophilic interaction/strong cation‐exchange stationary phase for capillary liquid chromatography and pressure‐assisted CECLin, J; Huang, G; Lin, X; Xie, Z
-
Microdeposition device interfacing capillary electrochromatography and microcolumn liquid chromatography with matrix‐assisted laser desorption/ionization mass spectrometryTegeler, TJ; Mechref, Y; Boraas, K; Reilly, JP; Novotny, MV
-
Capillary electrochromatography in anion exchange and normal‐phase mode using monolithic stationary phasesLammerhofer, M; Svec, F; Frechet, JMJ; Lindner, W
-
Preparation of a mixed‐mode hydrophilic interaction/anion‐exchange polymeric monolithic stationary phase for capillary liquid chromatography of polar analytesLin, J; Lin, J; Lin, X; Xie, Z
-
Rapid capillary electrochromatographic profiling of phytohormones on a hydrophilic interaction/strong anion‐exchange mixed‐mode monolithXucong, L; Yanping, L; Dongjie, X; Chunmei, Y; Zenghong, X
-
Chiral monolithic columns for enantioselective capillary electrochromatography prepared by copolymerization of a monomer with quinidine functionality. 1. Optimization of polymerization conditions, porous properties, and chemistry of the stationary phaseLammerhofer, M; Peters, EC; Yu, C; Svec, F; Frechet, JMJ
-
A facile versatile polymeric monolith for multiple separationsLin, X; Lin, J; Sun, Y; Li, Y; Xie, Z
-
Vinylbenzyl quaternary ammonium‐based polymeric monolith with hydrophilic interaction/strong anion exchange mixed‐mode for pressurized capillary electrochromatographyLin, X; Feng, S; Jia, W; Ding, K; Xie, Z
-
Capillary electrochromatography with monolithic stationary phases. II. Preparation of cationic stearyl‐acrylate monoliths and their electrochromatographic characterizationBedair, M; El Rassi, Z
-
Novel zwitterionic polyphosphorylcholine monolithic column for hydrophilic interaction chromatographyJiang, Z; Reilly, J; Everatt, B; Smith, NW
-
Novel highly hydrophilic zwitterionic monolithic column for hydrophilic interaction chromatographyJiang, Z; Smith, NW; Ferguson, PD; Taylor, MR
-
Synthesis of porous zwitterionic sulfobetaine monoliths and characterization of their interaction with proteinsViklund, C; Irgum, K
-
Preparation and evaluation of a sulfoalkylbetaine‐based zwitterionic monolithic column for CEC of polar analytesWang, X; Lin, X; Xie, Z
-
Zwitterionic polymeric monoliths for HILIC/RP mixed mode for CEC separation applicationsGuerrouache, M; Pantazaki, A; Millot, MC; Carbonnier, B
-
Recent advances in nonpolar and polar organic monoliths for HPLC and CECJonnada, M; Rathnasekara, R; El Rassi, Z
-
Preparation of continuous beds for electrochromatography and reversed‐phase liquid chromatography of low‐molecular‐mass compoundsEricson, C; Liao, JL; Nakazato, K; Hjerten, S
-
Exchange HPLC of proteins using porous poly(glycidyl methacrylate‐Co‐ethylene dimethacrylate) monoliths grafted with poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid)Viklund, C; Svec, F; Frechet, JMJ; Irgum, K
-
Polymethacrylate monolithic columns for capillary liquid chromatographyUrban, J; Jandera, P
-
Preparation of polymethacrylate monolithic stationary phases having bonded octadecyl ligands and sulfonate groups: Electrochromatographic characterization and application to the separation of polar solutes for pressurized capillary electrochromatographyLin, J; Wu, X; Lin, X; Xie, ZH
-
Controlling the surface chemistry and chromatography properties of methyacrylate‐ester‐based monolithic capillary columns via photograftingEeltink, S; Hilde, EF; Geiser, L; Svec, F; Frechet, JMJ; Rozing, GP
-
In situ functionalization of N‐acryloxysuccinimide‐based monolith for reversed‐phase electrochromatographyGuerrouache, M; Carbonnier, B; Vidal‐Madjar, C; Millot, MC
-
Hydrophilic diol monolith for the preparation of immuno‐sorbents at reduced nonspecific interactionsGunasena, D; El Rassi, Z
-
Neutral, charged and stratified polar monoliths for hydrophilic interaction capillary electrochromatographyGunasena, DN; El Rassi, Z
-
Targeting human serum fucome by an integrated liquid‐phase multicolumn platform operating in “cascade” to facilitate comparative mass spectrometric analysis of disease‐free and breast cancer seraSelvaraju, S; El Rassi, Z
-
Postpolymerization modification of a hydroxy monolith precursor. Part I. Epoxy alkane and octadecyl isocyanate modified poly (hydroxyethyl methacrylate‐Co‐pentaerythritol triacrylate) monolithic capillary columns for reversed‐phase capillary electrochromatographyShantipriya, K; El Rassi, Z
-
Post polymerization modification of a hydroxy monolith precursor. Part II. Epoxy biphenyl modified poly(hydroxyethyl methacrylate‐Co‐pentaerythritol triacrylate) monolithic capillary columns for reversed‐phase capillary electrochromatography based on π‐π and hydrophobic interactionsShantipriya, K; El Rassi, Z
-
Postpolymerization modification of a hydroxy monolith precursor. Part III. Activation of poly(hydroxyethyl methacrylate‐Co‐pentaerythritol triacrylate) monolith with epoxy functionalities followed by bonding of glycerol, polyamines, and hydroxypropyl‐β‐cyclodextrin for hydrophilic interaction and chiral capillary electrochromatographyShantipriya, K; El Rassi, Z
-
Preparation of an electrochromatographic stationary phase using a new polymethacrylate monolith with chloropropyl functionalityGökaltun, A; Aydoğan, C; Celebi, B; Denizli, A; Tuncel, A
-
A new zwitterionic electrochromatographic stationary phase based on poly(3‐chloro‐2‐hydroxypropyl methacrylate‐Co‐ethylene dimethacrylate) reactive monolithAydoğan, C; Çelebi, B; Bayraktar, A; Yılmaz, F; Denizli, A; Tuncel, A
-
Preparation and evaluation of a neutral methacrylate‐based monolithic column for hydrophilic interaction stationary phase by pressurized capillary electrochromatographyWang, X; Lin, X; Xie, Z; Giesy, JP
-
The challenges of the analysis of basic compounds by high performance liquid chromatography: some possible approaches for improved separationsDavid, V; Calley, M
-
CEC with new monolithic stationary phase based on a fluorinated monomer, trifluoroethyl methacrylateYurtsever, A; Saracoglu, B; Tuncel, A
-
Molded rigid polymer monoliths as separation media for capillary electrochromatography. 2. Effect of chromatographic conditions on the separationPeters, EC; Petro, M; Svec, F; Frechet, JMJ
-
Preparation and evaluation of the highly cross‐linked poly(1‐hexadecane‐co‐trimethylolpropane trimethacrylate) monolithic column for capillary electrochromatographyMinghua, L; Feng, Q; Lu, Q; Cai, Z; Zhang, L; Chen, G
-
Electrochromatography on acrylate‐based monolith in cyclic olefin copolymer microchip: a cost‐effective and easy‐to‐use technologyLadner, Y; Cretier, G; Faure, K
-
“Thiol‐ene” click chemistry: a facile and versatile route for the functionalization of porous polymer monolithsLv, Y; Lin, Z; Svec, F
-
Capillary electrochromatography with monolithic stationary phases: 1. Preparation of sulfonated stearyl acrylate monoliths and their electrochromatographic characterization with neutral and charged solutesBedair, M; El Rassi, Z
-
Preparation and characterization of hexyl methacrylate monolithic columns for CECCanto‐Mirapeix, A; Herrero‐Martinez, JM; Mongay‐Fernandez, C; Simo‐Alfonso, EF
-
Preparation and evaluation of lauryl methacrylate monoliths with embedded silver nanoparticles for capillary electrochromatographyNavarro‐Pascual‐Ahuir, M; Lerma‐Garcia, MJ; Ramis‐Ramos, G; Simo‐Alfonso, EF; Herrero‐Martinez, JM
-
Novel highly hydrophilic methacrylate based monolithic column with mixed‐mode of hydrophilic and strong cation‐exchange interactions for pressurized capillary electrochromatographyLin, J; Liu, S; Lin, J; Lin, X; Xie, Z
-
Simultaneous determination of nucleoside and purine compounds in human urine?based on a hydrophobic monolithic column using capillary electrochromatographyChen, Z; Huang, C; Liu, W; Zhang, L; Tong, P; Zhang, L
-
Macroporous polyacrylamide poly(ethylene glycol) matrixes as stationary phases in capillary electrochromatographyPalm, A; Novotny, MV
-
Performance of a monolithic silica column in a capillary under pressure‐driven and electrodriven conditionsIshizuka, N; Minakuchi, H; Nakanishi, K
@Phil_Robichaud
@deepthiw
@JoseServera