Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe

Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe Summary. In pigs, the gene for glucosephosphate isomerase (GPI) is linked to the halothane (HAL) gene which is responsible for malignant hyperthermia (MH). A single copy DNA probe, designated GPI8R, has been isolated from a pig genomic library using a porcine GPI cDNA probe. This probe detects, as was the case for the cDNA probe, a five allele polymorphism in Sac1 andPvuII digested pig DNA. Family studies show that this polymorphism is linked to the HAL locus and hence can be used in carrier detection. In situ hybridization with GPI8R assigned the GPI locus to bands p12‐q22 of chromosome 6. We conclude that the HAL linkage group resides on chromosome 6. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Animal Genetics Wiley

Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe

Loading next page...
 
/lp/wiley/porcine-malignant-hyperthermia-carrier-detection-and-chromosomal-QJjY1IHwLI
Publisher
Wiley
Copyright
© 1988 International Society for Animal Genetics
ISSN
0268-9146
eISSN
1365-2052
D.O.I.
10.1111/j.1365-2052.1988.tb00809.x
Publisher site
See Article on Publisher Site

Abstract

Summary. In pigs, the gene for glucosephosphate isomerase (GPI) is linked to the halothane (HAL) gene which is responsible for malignant hyperthermia (MH). A single copy DNA probe, designated GPI8R, has been isolated from a pig genomic library using a porcine GPI cDNA probe. This probe detects, as was the case for the cDNA probe, a five allele polymorphism in Sac1 andPvuII digested pig DNA. Family studies show that this polymorphism is linked to the HAL locus and hence can be used in carrier detection. In situ hybridization with GPI8R assigned the GPI locus to bands p12‐q22 of chromosome 6. We conclude that the HAL linkage group resides on chromosome 6.

Journal

Animal GeneticsWiley

Published: Jun 1, 1988

References

  • A partial cDNA clone for porcine glucosephosphate isomerase: isolation, characterization and use in detection of restriction fragment length polymorphisms
    Davies, Davies; Harbitz, Harbitz; Hauge, Hauge
  • Tentative chromosomal localization of the bovine major histocompatibility complex by in situ hybridization
    Fries, Fries; Hediger, Hediger; Stranzinger, Stranzinger
  • DNA polymorphism and human disease
    Gusella, Gusella

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off